地黄多糖干预对多次低剂量X线照射小鼠精子DNA完整性的影响  

Effects of Rehmannia Glutinosa polysaccharide intervention on the sperm DNA integrity in mouse exposed to repeated low-dose X-ray irradiation

作　　者：仵春云[1] 张杜平[2] 赵昌涛[2] 唐开发[3] 邢俊平[2] 

机构地区：[1]西安交通大学第一附属医院预防保健科西安710061 [2]西安交通大学第一附属医院泌尿外科 [3]贵阳医学院附属医院泌尿外科

出　　处：《中国男科学杂志》2015年第12期22-25,31,共5页Chinese Journal of Andrology

基　　金：陕西省科技计划项目(编号:2008K-15)

摘　　要：目的：探讨地黄多糖干预对X线照射小鼠精子DNA完整性的影响。方法选择雄性昆明小鼠70只（5~6周龄；体重22±2g），随机分为A组（单纯照射组21只）、B组（照射＋200mg/Kg RPS灌胃组21只）、C组（照射＋800mg/Kg RPS灌胃组21只）、D组（对照组7只）；再将A、B、C组分别随机分为3组，每组7只。所有小鼠在照射前5天开始每天灌胃1次，其中A组及D组给予0.2mL生理盐水灌胃，B组及C组分别给予200mg/Kg、800mg/Kg地黄多糖溶于0.2mL生理盐水灌胃，共灌胃24次。A、B、C组采用低剂量X线每天1次全身照射，共15次；单次照射剂量分别为0.025Gy、0.075Gy、0.100Gy，剂量率为0.025Gy/min。所有小鼠在末次照射结束后48h内脱颈法处死，立即收集附睾中精子并通过单细胞凝胶电泳（彗星试验）检测精子DNA的完整性。结果单纯X线照射后造成精子DNA损伤，其严重程度随剂量增加而加重。给予200mg/Kg及800mg/Kg地黄多糖灌胃则均能减轻低剂量X线照射对小鼠精子DNA的损伤，且后者较前者作用更明显，两者具有统计学意义（P＜0.05）。结论低剂量X线多次全身照射可引起小鼠精子DNA损伤，并呈剂量依赖性，不同剂量地黄多糖对X线照射后的小鼠精子DNA完整性具有保护作用。Objective To explore the effects of Rehmannia polysaccharide intervention on the sperm DNA integrity in mouse exposed to repeated X-ray irradiation.Methods Total of 70 male mice （5~6 weeks old; weight 22 ±2g） were randomly divided into four groups such as group A （irradiation group 21 mice）, group B （radiation and 200mg/Kg RPS fed group, 21 mice） , group C （radiation and 800mg/Kg RPS fed group 21 mice）, group D （control group 7 mice）; and then mice in group A, B, C were subdivided into 3 groups, respectively and each group had 7 mice. During d 5 days before irradiation, mice in groups of A and D were intragastrically administrated with 0.2ml saline, group B and group C were intragastrically administrated with 200mg/Kg, 800mg/Kg Rehmannia Glutinosa Polysaccharide which were dissolved in 0.2mL saline, and intragastric administration was done 24 times. Mice in group A, B and C was treated with low-dose whole body X-irradiation for 15 times; the single irradiation doses were 0.025, 0.075 and 0.100Gy with dose rate 0.025Gy/min. Collected sperm in epididymis immediately in 48 hours after irradiation from all mice. The integrity of sperm DNA was detected by the line comet assay （SCGE）.Results With increasing doses of X-ray irradiation, the damage of sperm DNA increased. 200mg/Kg and 800mg/Kg of Rehmannia Glutinosa Polysaccharide given intragastrically both reduce the damage sperm DNA which caused by low-dose X-ray radiation, the effect of latter is more obvious than that of the former, both statistically significant（P〈0.05）.Conclusion Repeated low-dose whole body X-irradiation can result in the damage of sperm DNA and the damage increased gradually as the doses increasing. Rehmannia Glutinosa Polysaccharide may protect the integrity of sperm DNA.

关 键 词：辐射剂量 地黄 精子 DNA损伤 

分 类 号：R358.4[医药卫生—基础医学]