基于反转录双链DNA扩增片段长度多态性技术的不同产地太子参基因差异表达分析  

Analysis of Gene Expression of Pseudostellariae Radix from Different Habitats Based on cDNA-AFLP Analysis

作　　者：华愉教 耿超[1] 王胜男[1] 刘训红[1] 谷巍[1] 罗益远 刘娟秀[1] 侯娅[1] 马阳[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中国药学杂志》2016年第4期269-273,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81274016;81473312);江苏高校优势学科建设工程资助项目(ysxk-2014)

摘　　要：目的研究不同产地太子参的基因差异表达,分离并鉴定相关差异基因。方法采用反转录双链DNA扩增片段长度多态性技术分析国内4个主产地太子参差异表达基因片段。结果筛选6个引物组合进行扩增,从不同产地太子参中获得34条差异表达转录衍生片段(trivially distributed file system,TDFs),对差异片段进行克隆、序列测定,得到21个TDFs核苷酸序列。经过BLASTX程序比对,15个TDFs有其对应的显著同源序列,其中7个TDFs为已知功能的蛋白,这些蛋白主要参与植物的生长发育、抵御病虫害、提高植物抗非生物胁迫的能力。结论利用c DNA-AFLP技术鉴定出不同产地太子参的差异基因,为揭示太子参药材品质形成的分子机制提供基础资料。OBJECTIVE To investigate the gene differential expression in Pesudostellariae Radix from different habitats, isolate and evaluate associated differential genes. METHODS cDNA-AFLP technique was applied to analyze the differential expression genes of Pesudostellariae Radix from four different habitats. RESULTS Six primer pairs were selected and amplified. Thirty-four differentially expressed trivially distributed file system （TDFs） were obtained from Pesudostellariae Radix samples from different habitats. Then these TDFs were cloned and sequenced, and the nucleotide sequences of 21 TDFs were obtained. By BLASTX analysis, 15 of them were found to have homologous sequences in the databases, among which seven TDFs had known function. These proteins were mainly involved in the growth and development of plants and played a role in the metabolisms of defending diseases and insect pests, and improving the ability of plants to resist abiotic stress. CONCLUSION This research evaluates differential expression genes in Pesudostellariae Radix from different habitats, providing the basic information for revealing the molecular mechanism of the property formation of Pesudostellariae Radix.

关 键 词：太子参 不同产地 反转录双链DNA扩增片段长度多态性 差异表达基因 

分 类 号：R284[医药卫生—中药学]