壳聚糖/Fe_3O_4/聚(N-异丙基丙烯酰胺-co-丙烯酸)/大肠杆菌复合微囊的制备与表征  

Preparation and characterization of chitosan/Fe_3O_4/poly(N-isopropylacrylamide-co-acrylic acid)/escherichia coli composite microcapsule

作　　者：宫艺[1] 孙敏敏[1] 朱爱梅[1] 刘庆林[1] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005

出　　处：《中国科技论文》2015年第24期2847-2849,共3页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20130121130006)

摘　　要：为了保持细胞的生物活性,基于皮克林乳液开发了条件温和的细胞包裹技术,成功地制备了壳聚糖/Fe3O4/聚(N-异丙基丙烯酰胺-co-丙烯酸)/大肠杆菌复合微囊。首先采用微波辅助分散聚合法和共沉淀法分别制备了聚(N-异丙基丙烯酰胺-co-丙烯酸)(P(NIPAM-co-AAc))微凝胶与油酸改性的Fe3O4纳米粒子,并用其作为稳定剂制备皮克林乳液。最后大肠杆菌(E.coli)加入水相后分散在乳液液滴内部,使用异硫氰酸荧光素标记的壳聚糖(FITC-CS)交联油水界面的Fe3O4与P(NIPAMAAc)微凝胶形成微囊。通过扫描电镜、透射电镜和激光共聚焦荧光显微镜(LCSM)对制备的凝胶、纳米粒子和复合微囊的形貌结构和组成进行了观察和分析。In order to maintain cell biological activity,the cell encapsulation with wild condition was developed based on Pickering emulsion,the Chitosan/Fe3O4/poly（N-isopropylacrylamide-co-acrylic acid）/Escherichia coli composite microcapsule was prepared and characterized.The P（NIPAM-co-AAc）microgels and oleic acid-modified Fe3O4 nanoparticles were prepared by microwaveassisted dispersion polymerization and co-precipitation method,respectively,and then they were used as the stabilizer to fabricate Pickering emulsion.Chitosan was used to crosslink the P（NIPAM-co-AAc）microgels and Fe3O4 nanoparticles on the interface of the emulsion droplets which leads to the formation of novel organic/inorganic/biological composite microcapsule.Laser confocal scanning fluorescence microscopy（LCSM）was used to identify emulsion type of the Pickering emulsion.The morphology and structure of the composite microcapsule was observed by SEM and LCSM.

关 键 词：复合微囊 细胞包裹 大肠杆菌 

分 类 号：TB33[一般工业技术—材料科学与工程]