PTSD与BDNF基因Val66Met多态性相互作用对青少年血脂的影响  

Effects of interactions between post-traumatic stress disorder with brain-derived neurotrophic factor gene Val66Met polymorphism on serum lipid profiles in adolescents

作　　者：樊梅[1] 李蓉晖[1] 胡敏珊[1] 方定志[1] 

机构地区：[1]四川大学华西基础医学与法医学院,成都610041

出　　处：《中国科技论文》2015年第24期2874-2879,共6页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20130181110026)

摘　　要：为验证"创伤后应激障碍(post-traumatic stress disorder,PTSD)和脑源性神经营养因子(brain derived neurotrophic factor,BDNF)基因BDNF Val66Met多态性之间存在相互作用并影响血脂及相关指标"的新假设,以2008年汶川地震灾区高中学生为研究对象,采用PTSD检查量表平民版(PTSD checklist-civilian version,PCL-C)评估PTSD症状,常规体格检查并计算体质指数(body mass index,BMI)和腰臀比(waist-hip ratio,WHR),常规方法测定血清总胆固醇(total cholesterol,TC),甘油三酯(triglyceride,TG),低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C),血糖,聚合酶链反应-限制性酶切法(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)分析BDNF Val66Met多态性,DNA测序验证。发现Val/Val基因型男性PTSD组TG显著高于对照组,PTSD组Val/Val基因型男性TG显著高于Met等位基因携带者。携带Met等位基因女性PTSD组BMI显著高于对照组。结果表明,PTSD与BDNF Val66Met多态性在不同性别青少年中存在不同的相互作用,并影响血脂及相关指标水平。本研究为深入探讨影响血脂及相关指标的因素及其机制提供了新线索,为脂蛋白异常血症、心血管疾病的精准医学防治或个性化防治提供了新的思路。To test our hypothesis that the interplay may occur between post-traumatic stress disorder（PTSD）and brain-derived neurotrophic factor（BDNF）gene BDNF Val66 Met polymorphism and affect serum lipid profiles.Chinese high school students were enrolled after the 2008 Wenchuan earthquake.The PTSD checklist-civilian version（PCL-C）was used to measure the symptoms of PTSD.Body mass index（BMI）and waist-hip ratio（WHR）were calculated.Serum levels of total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）and glucose were tested by routine methods.BDNF Val66 Met polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）and verified by DNA sequencing.The results show that the male PTSD subjects have higher TG than male subjects without PTSD in the Val/Val homozygotes.Compared with the Met allele carriers,Val/Val homozygotes have higher TG in the males with PTSD.The female PTSD subjects have higher BMI than the female subjects without PTSD in the Met allele carriers.The results suggest that the changes of lipid profiles induced by interactions of PTSD and BDNF Val66 Met polymorphism are different in adolescents with different gender.These findings will provide new insights into further exploration of factors influencing lipid profiles and the mechanism,and precision medicine and personalized prevention of dyslipoproteinemia and cardiovascular diseases.

关 键 词：人体生物化学 精神病学 脑源性神经营养因子 基因多态性 创伤后应激障碍 血脂 

分 类 号：R749.5[医药卫生—神经病学与精神病学]