女贞子不同炮制品HPLC指纹图谱色谱峰的归属与比较  

Identification and Comparison of Constituents of Different Processed Products of Ligubtrum lucidum Fruit by HPLC Fingerprint

作　　者：梁晓[1] 吴鹏[1] 张学兰[1] 李转梅 王均秀 张群群[1] 孟艳[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中药材》2015年第11期2288-2292,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81373968)

摘　　要：目的:对女贞子不同炮制品HPLC指纹图谱色谱峰进行归属和比较,探讨女贞子炮制前后化学成分的变化规律。方法:采用HPLC法,以乙腈-0.1%甲酸溶液为流动相梯度洗脱,比较女贞子生品、酒蒸品和清蒸品在240、280 nm波长下指纹图谱色谱峰数目和峰面积变化,用已知对照品为参照对各色谱峰进行归属。结果:女贞子生品检出40个色谱峰,归属18个色谱峰,包括11个环烯醚萜类成分,5个苯乙醇类成分,1个黄酮类成分,1个醛类成分。女贞子酒蒸品和清蒸品均检出38个色谱峰,归属15个色谱峰,包括7个环烯醚萜类成分,5个苯乙醇类成分,1个黄酮类成分,1个醛类成分,1个有机酸类成分。女贞子生品与两种蒸制品指纹图谱整体图貌具有显著差异,而酒蒸品与清蒸品指纹图谱差别不大。结论:女贞子经酒蒸或清蒸后,其化学成分的组成及含量变化显著,而酒蒸品与清蒸品主要化学成分组成及其含量较为相近。Objective： To identify and compare the constituents in different processed products of Ligubtrum lucidum fruit by HPLC fingerprint,in order to study the changes of chemical constituents before and after processing. Methods： HPLC analysis was carried out with the mobile phase of acetonitrile and 0. 1% formic acid in a gradient elution mode. The number of HPLC fingerprint chromatographic peaks and peak area changes in two processed products and the crude product under 240 nm and 280 nm were compared. Then the obtained fingerprint chromatographic peaks were confirmed according to the the standard references. Results： 40 chromatographic peaks were detected from Ligustrum lucidum fruit,of which 18 chromatographic peaks were confirmed,including eleven iridoids,five phenethyl alcohols,one flavonoid and one aldehyde. 38 chromatographic peaks were detected from both steaming with wine product and the steamed product,of which 15 chromatographic peaks were confirmed,including seven iridoids,five phenethyl alcohols,one flavonoid,one aldehyde and one organic acid. There was a significant difference of fingerprint among crude Ligustrum lucidum fruit and its two processed products,but little difference between steaming with wine product and the steamed product. Conclusion： HPLC fingerprint of the steaming with wine product and the steamed product of Ligustrum lucidum fruit are similar while the changes on chemical composition and the content in steaming with wine product and steamed product of Ligustrum lucidum fruit are remarkable.

关 键 词：女贞子 炮制品 HPLC指纹图谱 色谱峰归属 

分 类 号：R283.1[医药卫生—中药学]