超临界CO_2萃取与水蒸气蒸馏提取疏柔毛变种罗勒挥发油成分的比较研究  

Comparison of Chemical Components of Essential Oil from Ocimum basilicum var. pilosum Extracted by Supercritical CO_2 Fluid and Steam Distillation

作　　者：王兆玉[1] 郑家欢[1] 施胜英 罗志雄[1] 倪舜宇 林敬明[2] 

机构地区：[1]广东药学院生命科学与生物制药学院/广东省生物技术候选药物研究重点实验室,广东广州510006 [2]南方医科大学珠江医院药剂科,广东广州510282

出　　处：《中药材》2015年第11期2327-2330,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31201236);广东科技厅科技引导项目计划(2012A030100008);广东省自然科学基金(S2013010014796);广东省中医药局科研基金(20131244)

摘　　要：目的:比较超临界CO_2萃取法(SFE-CO_2)和水蒸气蒸馏法(SD)提取疏柔毛变种罗勒挥发油化学成分的差异。方法:采用SFE-CO_2法和SD法提取疏柔毛变种罗勒的挥发油,用GC-MS分析鉴定挥发油的化学成分,用归一化法测定各化合物的相对百分含量,比较两种不同方法所提挥发油成分的差异。结果:从SFE-CO_2法提取的挥发油中鉴定了42种成分,从SD法提取的挥发油中鉴定了40种成分,两者共有成分为25种。结论:不同提取方法对疏毛变种罗勒挥发性成分的提取效率不同,在现代化中药制剂生产过程中可采用多种方法联合提取有效成分。Objective： To compare the chemical components of essential oil prepared by steam distillation extraction（ SD） and supercritical CO2 fluid extraction（ SFE-CO2） from Ocimum basilicum var. pilosum whole plant. Methods： The essential oil of Ocimum basilicum var. pilosum were extracted by SD and SFE-CO2. The chemical components of essential oil were separated and analyzed by gas chromatography-mass spectrometry（ GC-MS）. Their relative contents were determined by normalization of peak area. Results： 40 and 42 compounds were detected in the essential oil prepared by SD and SFE-CO2 respectively. 25 compounds were common. Conclusion： There are significant differences of the chemical components between the Ocimum basilicum var. pilosum essential oil prepared by SD and that by SFE-CO2. Different methods showed different extraction efficiency with a special compound. It might be a good idea to unite several methods in the modern traditional Chinese medicine industry.

关 键 词：疏柔毛变种罗勒 挥发油 超临界CO2萃取 水蒸气蒸馏 GC-MS 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]