基于生物网络的当归芍药散、桂枝茯苓丸治疗原发性痛经作用机制研究  

Mechanism Study on Danggui Shaoyao San and Guizhi Fuling Wan For Treating Primary Dysmenorrheal Based on Biological Network

作　　者：王运来[1] 胡虒尧 方庆[1] 李国强[1] 潘永福[1] 姚瑶[1] 许钒[1] 

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]中国药科大学,江苏南京210009

出　　处：《中药材》2015年第11期2348-2352,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81173368)

摘　　要：目的:探索当归芍药散和桂枝茯苓丸治疗肝郁肾亏、气滞血瘀型原发性痛经的作用机制。方法:收集Pub Med和Gene Card数据库中复方、证型、原发性痛经的靶点信息,构建并分析病-证、病-证-方复合网络,计算机分子对接方法对复方所含成分雌激素样作用加以验证。结果:病-证-方网络共享多个靶点,涉及白细胞介素、前列腺素2α等多种重要节点。当归芍药散主要影响中枢促肾上腺皮质素释放激素等激素的释放,桂枝茯苓丸则通过影响外周环氧化酶所致的一系列炎症以及凝血相关靶点信息达到治疗作用。分子对接结果提示当归芍药散的类雌激素样作用优于桂枝茯苓丸。结论:生物网络方法能在一定程度上解释两首复方作用于不同证型原发性痛经的分子机制。Objective： To investigate the molecular mechanisms of Danggui Shaoyao San and Guizhi Fuling Wan for treating primary dysmenorrhea of liver depression and blood stasis syndrome by biological network method. Methods： Targets of the formula were collected from Pub Med database,and targets of primary dysmenorrhea were searched from Gene Card database. Then,the ＂formula-syndromes-disease＂network was constructed and analyzed with Cytoscape software. Molecular docking approach was used to verify estrogen-like effect of ingredients. Results： The ＂formula-syndromes-disease＂shared multiple targets,which involves a variety of important nodes,such as IL and PGF2α,and played the effects of anti-inflammatory and analgesic. Danggui Shaoyao San could influence the function of hormones,such as corticotropin releasing hormone,and inhibit hyperactivity of adrenal axis. Guizhi Fuling Wan mainly affected a series of inflammation caused by cyclooxygenase and had the effects of blood coagulation. Estrogen-like effect of Danggui Shaoyao San was stronger than that of Guizhi Fuling Wan. Conclusion： The novel approach will present an effective strategy for theory study of Danggui Shaoyao San and Guizhi Fuling Wan for treating primary dysmenorrhea.

关 键 词：生物网络 原发性痛经 当归芍药散 桂枝茯苓丸 

分 类 号：R285.5[医药卫生—中药学]