人桥本甲状腺炎甲状腺组织中miRNAs表达谱的差异性分析  

People Hashimoto Thyroiditis miRNAs in Thyroid Organization Express the Differences of Spectral Analysis

作　　者：张顺霞[1] 李淑霞[2] 王江 陆勤 姜韬[5] 

机构地区：[1]宁夏大学校医院,宁夏银川750002 [2]宁夏银川市第一人民医院,宁夏银川750001 [3]三门峡人民医院,河南三门峡472000 [4]宁夏银川市妇幼保健院,宁夏银川750001 [5]宁夏医科大学总医院结直肠外科,宁夏银川750004

出　　处：《现代生物医学进展》2015年第36期7171-7174,共4页Progress in Modern Biomedicine

基　　金：宁夏自然科学基金项目(NZ13038)

摘　　要：目的:桥本甲状腺炎是一种自身免疫性甲状腺疾病,且其发病率呈逐年上升趋势,在医疗实践中,HT被认为是原发性甲状腺功能减退最常见的原因,同时较易发生甲状腺癌和淋巴瘤。另外,HT缺乏早期诊断标准,临床上发病隐匿且表现多样,病人多不易察觉而延误治疗。本文旨在应用micro RNA芯片技术系统筛查HT病变甲状腺组织,以此研究并揭示其HT的miRNA表达谱变化。方法:本研究首先采用microRNA芯片技术,对正常甲状腺组织及桥本甲状腺炎甲状腺组织中micro RNA的表达进行比较,筛选桥本甲状腺炎中差异性表达的miRNAs。结果:与正常甲状腺相比,在桥本甲状腺炎及其合并甲状腺乳头状癌的一侧桥本甲状腺炎中分别有39个和25个miRNAs分子发生了差异性表达(P<0.05),比较2组中共有的miRNAs发现,miR-142-3p、miR-338-3p、miR-454、miR-146a、miR-29b-1*、miR-150、miR-223表达上调,miR-654-5p、miR-601、miR-198、miR-1226*表达下调(log2 FC≥2,P<0.05);而miR-142-5p在原发性桥本甲状腺炎中表达显著性升高近8倍(log2 FC=7.959,P<0.01)。结论:我们通过micro RNA芯片,首次直接系统筛查了桥本甲状腺炎病变组织相关miRNA表达谱,总体上初步掌握了与正常甲状腺相比,桥本甲状腺炎及合并甲状腺乳头状癌时其miRNA表达谱的变化情况,为我们后续的研究提供了方向与基础。Objective： Hashimoto thyroiditis is a kind of autoimmune thyroid disease, and there is an upward trend in the incidence of this disease. In medical practice, HT is believed to be the most common cause of primary hypothyroidism and is considered to be related to development of both thyroid carcinoma and lymphoma. Because of the lack of early diagnostic criteria of HT, and its diversity and performance is too difficult to detect, the patient always suffer more delay treatment with poor outcomes. This paper aims to screen the thyroid tissue of HT lesions and reveal the changes of miRNA expressions of HT by microRNA microarray technology system.Methods： With miRNA array, we compared miRNA expressions of different normal thyroid tissue with HT. Results： The results of miRNA array showed that, compared with normal thyroid, there had been 39 and 25 miRNAs different expression in HT and HT side of HT association with papillary thyroid carcinoma（PTC）, respectively（P〈0.05）; miR-142-3p, miR-338-3p, miR-454, miR-146 a,miR-29b-1＊, miR-150 and miR-223 got the same miRNAs up-regulation（log2 FC≥2,P〈0.05）. while miR-654-5p, miR-601, miR-198 and miR-1226＊（log2 FC≥2, P〈0.05）got down-regulation. In addition,miR-142-5p increased nearly eight times higher in primary HT than the normal thyroid（log2 FC =7.959, P〈0.01）. Conclusions： With the method of micro RNA microarray, we found the related miRNA expression profiles of the Hashimoto＇s thyroiditis, general initially mastered and compared the miRNA expression profiles of Hashimoto＇s thyroiditis with normal thyroid, which laid a foundation for the further functional research of the disease-associated miRNAs.

关 键 词：桥本甲状腺炎 microRNA芯片 差异性 

分 类 号：R581[医药卫生—内分泌]