钼对小鼠免疫球蛋白和细胞因子含量的影响  

Effect of molybdenum on the immunoglobulin and cytokine contents in mouse

作　　者：赵红先 杨自军[1] 杜伯强[1] 田丽萍[1] 李爱强[1] 王业鑫[1] 张才[1] 汪纪仓[1] 

机构地区：[1]河南科技大学动物科技学院环境与畜产品安全河南省高校重点学科开放实验室,河南洛阳471003

出　　处：《中国兽医学报》2016年第3期485-489,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31040081)

摘　　要：旨在探讨钼对小鼠血清免疫球蛋白和细胞因子含量的影响,选用30d健康昆明小鼠60只,随机分为4组,分别饮用钼水(mg/L),Ⅰ组(0)、Ⅱ组(400)、Ⅲ组(800)、Ⅳ组(1 200),试验周期42d,定期剖杀取样。采用ELISA法检测小鼠血清中免疫球蛋白和细胞因子含量的变化,结果显示:与Ⅰ组相比,整个试验中,Ⅱ、Ⅲ、Ⅳ组小鼠血清IgE含量差异不显著(P>0.05),第14天Ⅲ、Ⅳ组IgG、IL-4均极显著降低(P<0.01),Ⅲ组IFN-γ、IL-6均显著升高(P<0.05),第28天Ⅱ、Ⅲ、Ⅳ组IgG,Ⅲ、Ⅳ组IgM、IL-4,Ⅳ组IL-2均显著或极显著降低(P<0.05或P<0.01),第42天Ⅱ、Ⅲ、Ⅳ组IgG、IL-2、IL-4均显著或极显著降低(P<0.05或P<0.01),且小鼠血清IgG、IgM、IL-2、IL-4含量随时间的延长呈下降趋势,IFN-γ、IL-6含量随时间的延长先上升后下降。结果表明:饮水中钼(≥400mg/L)可引起小鼠血清免疫球蛋白、抗炎细胞因子下降,促炎细胞因子先上升后下降,且具有一定的时-效和量-效关系,影响动物的体液免疫和细胞免疫功能。The experiment aimed to evaluate the effect of molybdenum on immunoglobulin and cytokine contents in mouse serum by the methods of ELISA.Four different doses of molybdenum（groupⅠ,0mg/L;groupⅡ,400mg/L;groupⅢ,800mg/L;groupⅣ,1 200mg/L）were added to the drinking water.60thirty-days-old mice were divided into four groups randomly for 42 days.Autopsies were made periodically to get the blood.The result showed that IgE in serum of mice were showed no significant change（P〉0.05）throughout the experiment.IgG and IgM in serum of mice were decreased with increasing the dose of molybdenum and exposure time.IL-2in serum of mice was significantly lower（P〈0.05 or P〈0.01）compared with that in groupⅠfrom 14 to 42d.IL-4in serum of mice were decreased with increasing the dose of molybdenum.IFN-γand IL-6in serum of mice was dropped after the first rise with the dose increasing.It is conclude that molybdenum over 400mg/L can decreasse the lever of immunoglobulin and anti-inflammatory cytokines and the contents to flammatory cytokines drop after the first rise.

关 键 词：钼 小鼠 免疫球蛋白 细胞因子 

分 类 号：S859.8[农业科学—临床兽医学]