中国西门塔尔牛AGPAT6基因外显子多态性及其与肌内脂肪酸含量的相关性  

Correlation analysis b etween the polymorphism of AGPAT6 gene exons and intramuscular fatty acid content in Chinese Simmental cattle

作　　者：龙小娟 房希碧[1] 姜平[1] 肖航[1] 芦春艳[2] 安兵兵[1] 赵志辉[1] 杨润军[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学农业实验基地,吉林长春130062

出　　处：《中国兽医学报》2016年第3期527-530,538,共5页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2013AA102505);转基因专项资助项目(2011ZX08007-001)

摘　　要：磷酸甘油酰基转移酶6(AGPAT6)是真核生物甘油三酯合成的关键酶,其在褐色脂肪组织、乳腺上皮细胞以及其他许多组织中高水平表达。为了研究AGPAT6基因与中国西门塔尔牛脂肪酸组成的相关性,本试验采用PCRRFLP的方法对AGPAT6基因的多态性进行检测,并将AGPAT6基因的遗传多态性位点与脂肪酸组成进行关联性分析。结果显示,在AGPAT6基因Exon1的303bp处有一个T>C的多态性位点,其中等位基因C为优势基因。在中国西门塔尔牛群体中携带CC基因型的肉牛个体具有较高十七碳一烯酸和较低的亚油酸含量(P<0.05)。本研究结果可为中国西门塔尔牛分子选育、进一步提高肉用性能提供试验依据。In eukaryotes,1-acylglycerol-3-phosphate-O-acyltransferases 6（AGPAT6）is a crucial enzyme for the triacylglycerol biosynthesis.And AGPAT6 gene is high expression in brown adipose tissue,mammary gland epithelial cells and many other tissue.To study the correlation between the polymorphism of AGPAT6 gene and fatty acid compositions in Chinese Simmental cattle,the PCRRFLP method was used to detect the polymorphism of AGPAT6 gene,and then the correlation between the locus containing polymorphism and the fatty acid compositions was analyzed by SPSS13.0software.The results showed that a mutation 303 bp T〉C in AGPAT6 gene exon1was detected,and E1-303TC showed a significant association with heptadecenoic acid（c17：1）and linoleic acid（c18：2n6c）compositions（P〈0.05）.The result provided the experimental basis for Chinese Simmental cattle breeding and production.

关 键 词：中国西门塔尔牛 AGPAT6基因 多态性 脂肪酸 

分 类 号：S813.3[农业科学—畜牧学]