PS/APP双转基因小鼠大脑中β-淀粉样蛋白的磁共振显微成像和组织切片研究  

Research of Magnetic Resonance Microscopy and Histological Section on Beta-Amyloid Plaques in PS/APP Transgenic Mice

作　　者：林岚[1] 付振荣[1] 张柏雯 周著黄[1] 高宏建[1] 吴水才[1] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124

出　　处：《中国医疗设备》2016年第2期31-33,38,共4页China Medical Devices

基　　金：北京市自然科学基金(7143171)资助

摘　　要：目的探讨双转基因小鼠PS/APP大脑三维磁共振显微成像(MRM)的T2图与二维组织切片中Aβ斑块之间的关系。方法本文提出了一个高效、全自动的图像处理流程,即先通过未染色的二维组织切片重建三维脑模型,之后将其与三维MRM数据配准。配准是一个多步的过程,首先对邻接的二维切片进行配准,然后再进行迭代的二维和三维配准。结果该流程可以较好配准组织切片与MRM,并能在T2图和染色切片上发现一定的Aβ斑块间的对应关系。结论通过神经影像技术来对转基因小鼠大脑中的Aβ斑块进行监测,了解阿尔茨海默症(AD)中Aβ斑块的产生、沉积和清除过程。Objective To investigate the relationship between magnetic resonance microscopy（MRM） T2 map associated with Aβ plaques and their histology on dual transgenic PS/APP mouse brain. Methods The paper proposed an efficient, automated image-processing pipeline that used unstained sections to reconstruct a 3D brain model from 2D histological sections and further registered these with the corresponding 3D MRM. The imaging registration was a multistep registration procedure that first aligned the neighboring 2D slices and then iteratively performed 2D and 3D registration. Results The registration pipeline can accurately register the 2D histology sections and 3D MRM. Some Aβ plaques can be found both in stained histology and MRM. Conclusion This research monitored the Aβ plaques in PS/APP transgenic mice through neuroimaging technology, which can facilitate the understanding of the generation, deposition, and removal of Aβ plaques in Alzheimer＇s disease.

关 键 词：Β-淀粉样蛋白 磁共振显微成像 阿尔茨海默病 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置]