Box-Behnken效应面法优化银线草中总黄酮的提取工艺  

Extraction Process Optimization of Total Flavonoids from Chloranthus Japonicus sieb by Box-Behnken Response Surface Methodology

作　　者：孙静[1] 张小飞[1] 刘洁琼[1] 贾天芊 

机构地区：[1]陕西中医药大学药物制剂教研室,陕西咸阳712000

出　　处：《中国药师》2016年第3期440-442,452,共4页China Pharmacist

基　　金：陕西省教育厅重点实验室项目(编号:11JS037)

摘　　要：目的:通过Box-Behnken效应面法优化银线草中总黄酮的提取工艺。方法:以乙醇浓度(X_1)、液料比(X_2)和提取时间(X_3)为自变量,以银线草中总黄酮的提取量(Y)为因变量,利用Box-Behnken效应面法优化银线草中总黄酮的提取工艺。结果:银线草中总黄酮的最佳提取工艺参数为:乙醇浓度为54.8%,液料比为13.6,提取时间为2.0 h,通过3批验证提取工艺参数,银线草中总黄酮的提取量实测值与回归模型预测值的相对误差为-2.34%。结论:使用Box-Behnken效应面法优化银线草中总黄酮的提取工艺,方法简便,精度更高。Objective： To optimize the extraction process of total flavonoids from Chloranthus japonicus sieb by Box-Behnken response surface methodology. Methods： The independent variables were the ethanol concentration（ X_1）,liquid-solid ratio（X_2） and extraction time（ X_3）,the dependent variable was the extraction amount of total flavonoids（Y）,and the extraction process was optimized by Box-Behnken response surface methodology. Results： The results showed that the optimal extraction conditions for total flavonoids from Chloranthus japonicus sieb were as follows： the ethanol concentration was 54. 8%,the liquid-solid ratio was 13. 6,and the extraction time was 2. 0 h. The verified extraction process parameters of three batches showed that the relative error between the experimental values and those predicted from the regression model was- 2. 34%. Conclusion： The optimal extraction process parameters are simple and more convenient with higher precision for the extraction of total flavonoids from Chloranthus japonicus sieb.

关 键 词：BOX-BEHNKEN效应面法 银线草 总黄酮 

分 类 号：R284.2[医药卫生—中药学]