3种中药提取物对人真皮成纤维细胞增殖及胶原合成的影响  

Effects of Three Herb Extracts on Cell Proliferation and Collagen Synthesis in Cultured Human Dermal Fibroblasts

作　　者：徐莲[1,2] 符旭东[1] 熊蕊[1,2] 罗梦颖 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北中医药大学药学院

出　　处：《中国药师》2016年第3期448-452,共5页China Pharmacist

摘　　要：目的:探讨3种中药提取物对体外培养人真皮成纤维细胞增殖及胶原合成的影响。方法:采用组织块法分离培养人真皮成纤维细胞,进行波形蛋白免疫组化鉴定。MTT法测定3种中药提取物作用后成纤维细胞的增殖能力,消化法检测培养液中羟脯氨酸的含量变化。结果:培养的细胞为典型的成纤维细胞形态,波形蛋白免疫组化呈阳性;冻存前后的人真皮成纤维细胞均具有较强的增殖能力;MTT检测结果显示培养72 h后,0.312 5~5.000 0 mg·ml^(-1)芦荟凝胶提取物、0.156 3~2.500 0 mg·ml^(-1)白芨提取物、0.075 0 mg·ml^(-1)丹参多酚酸盐,具有明显促进人成纤维细胞增殖作用(P<0.05,P<0.01),且培养上清液中羟脯氨酸含量高于空白对照组(P<0.01,P<0.05)。丹参多酚酸盐对成纤维细胞增殖的影响与时间和浓度关系密切,随着培养时间延长,高浓度药物组对成纤维细胞由促进变为抑制作用。结论:芦荟、白芨、丹参提取物可通过促进人真皮成纤维细胞增殖以及羟脯氨酸等细胞外基质的分泌加速创面愈合。Objective： To investigate the effects of three herb extracts on cell proliferation and collagen synthesis in cultured human dermal fibroblasts. Methods： The human dermal fibroblasts were isolated and cultured with tissue explant culture technique. Immunohistochemical staining was performed to observe vimentin in fibroblasts. The proliferation of fibroblasts was determined by MTT assay. Hydroxyproline in the cultural medium was determined by a digestion method. Results： The cells were typical fibroblasts,and the immunohistochemistry staining of vimentin in the cells was positive. The three herb extracts could promote the proliferation of the fibroblasts at appropriate concentrations in a time-dependent manner. After the 72-hour culture in the medium,0. 312 5-5. 000 0 mg·ml（-1）Aloe vera gel extract,0. 156 3-2. 500 0 mg·ml（-1）Bletilla striata extract and 0. 075 0 mg·ml（-1）salvianolate showed significant effects on the proliferation of fibroblasts（ P 〈0. 05 or P 〈0. 01 vs the blank group）. Compared with that in the blank group,the amount of hydroxyproline in the three herb extracts cultural medium was also increased（ P 〈0. 05 or P 〈0. 01）. The effect of salvianolate on the cell proliferation was closely related with time and concentration,and the high concentration group could inhibit the growth of fibroblasts as the extension of time. Conclusion： Aloe vera gel extract,bletilla striata extract and salvianolate can promote the proliferation of fibroblasts and the production of hydroxyproline,which may contribute to the wound healing.

关 键 词：芦荟凝胶提取物 白芨提取物 丹参多酚酸盐 成纤维细胞 细胞增殖 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]