ALA-PDT对尖锐湿疣凋亡及细胞因子的影响  

The effect of ALA-PDT of apoptosis and cytokine in the patients with cindyloma acuminate

作　　者：胡永轩[1] 谭妙娟 周冼苡[1] 吴中[1] 毛祖豪[1] 胡燕卿[1] 黄诗韵[1] 刘康兴[1] 卢艳[1] 韩雪[1] 

机构地区：[1]南方医科大学第三附属医院皮肤科,广州510630 [2]广东省食品药品职业技术学校,广州510663

出　　处：《临床与病理杂志》2016年第1期61-66,共6页Journal of Clinical and Pathological Research

基　　金：广州市天河区科技计划资助(201404KW007);广东省自然科学基金资助(2015A030310035)~~

摘　　要：目的:检测5-氨基酮戊酸-光动力疗法(ALA-PDT)治疗前后尖锐湿疣皮损凋亡相关因子(Fas、caspase3、Bcl-2)及促炎症细胞因子(CCL-20、TNF-α、IL-1)的表达,探讨AL A-PDT治疗尖锐湿疣的可能机理。方法:收集10例尖锐湿疣患者ALA-PDT治疗前后皮损(同时以正常健康者皮肤组织对照),采用荧光定量PCR检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA表达;Western blot检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1蛋白表达。结果:尖锐湿疣皮损组织内Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA和蛋白表达均高于正常皮肤组织(P<0.05);与治疗前比较,治疗后Bcl-2、CCL-20、TNF-α、IL-1 m RNA和蛋白表达水平明显降低(P<0.01),Fas、caspase3 m RNA和蛋白表达水平明显升高(P<0.01)。结论:AL A-PDT是一种能有效治疗尖锐湿疣的方法,它可诱导细胞表面凋亡蛋白Fas、caspase3的表达、减少凋亡抑制因子Bcl-2的表达,从而可能依靠内源性途径(线粒体介导的凋亡)和外源性途径(死亡受体介导的凋亡)促进尖锐湿疣角质形成细胞凋亡;并可能通过抑制促炎细胞因子CCL-20、TNF-α、IL-1而发挥局部免疫调节作用。Objective：To detect the expression of the apoptosis related factors（Fas,caspase3,Bcl-2） and cytokines（CCL-20,TNF-α,IL-1） in patients with condyloma acuminatum before and after the treatment with ALA-PDT,and explore the mechanism of ALA-PDT in the treatment of condyloma acuminatum.Methods：A total of 10 patients who were diagnosed with condyloma acuminatum were enrolled in this study and were treated by ALA-PDT for 3 or4 times.The lesions of condyloma acuminatum before and after the treatment（normal healthy skin tissues as the control）,were collected.The expression of Fas,caspase3,Bcl-2,CCL-20,TNF-α,IL-1 mRNA with Fluorescene quantitative reverse transcription polymerase chain reaction as well as the expression of Fas,caspase3,Bcl-2,CCL-20,TNF-α,IL-1 with Western blot were conducted.Results：The expression of Fas,caspase3,Bcl-2,CCL-20,TNF-α,IL-1 in condyloma acuminatum was higher than those in the normal skin tissues（P0.05）.Compared with before treatment with ALA-PDT,the expression of Bcl-2,CCL-20,TNF-α,IL-1 was significantly decreased after the treatment with ALA-PDT（P0.0l）,whereas the Fas,caspase3 expression was significantly increased（P0.0l）.Conclusion：ALA-PDT is an effective treatment for condyloma acuminatum.It can promote cell apoptosis in condyloma acuminatum by including cell surface apoptosis receptor Fas,caspase3 expression and inhibiting apoptosis inhibitory factor Bcl-2 expression.The specific apoptosis pathways include mitochondrion-mediated apoptosis（intrinsic pathway） and death receptor-mediated apoptosis（extrinsic pathway）.And it possibly plays a local immunomodulatory effect in condyloma acuminatum through inhibition of proinfiammatory cytokines CCL-20,TNF-α and IL-1.

关 键 词：尖锐湿疣 ALA-PDT 凋亡 细胞因子 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]