应用激光共聚焦显微扫描技术鉴别小麦矮腥黑穗病病菌和小麦光腥黑穗病病菌冬孢子  

Discrimination of teliospores in TCK and TFL with laser scanning confocal microscope

作　　者：蔚慧欣 高利[1] 康晓慧[2] 刘太国[1] 刘博[1] 陈万权[1] 

机构地区：[1]植物病虫害生物学国家重点实验室/中国农业科学院植物保护研究所,北京100193 [2]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《中国植保导刊》2016年第2期5-8,共4页China Plant Protection

基　　金：国家自然科学基金面上项目(31571965);小麦产业技术体系(CARS-03);北京市科技新星计划(Z131105000413057);北京市基金委与北科院联合资助项目(L140012)

摘　　要：应用激光共聚焦扫描显微术（CLSM）对小麦矮腥黑穗病病菌（TCK）和小麦光腥黑穗病病菌（TFL）冬孢子的自发荧光特性进行了研究。结果表明,两种冬孢子各自都含有自发荧光物质,均能在488 nm激发光下,分别在510-550 nm、530-600 nm中得到相应的发射光,且呈现的荧光分布均匀,大体形态清晰;而层切扫描发现自发荧光在这两种冬孢子的空间分布存在明显差异：TCK冬孢子的自发荧光物质主要分布在外孢壁和网脊上,而在原生质中分布少;TFL冬孢子的自发荧光物质主要分布在孢壁,原生质中分布少。Autofluorescence of teliosporesin Tillctia controversa（TCK） and Tillctia foeyida（TFL） was observed with laser scanning confocal microscope（LSCM）. The results showed that both of teliospores in TCK and TFL had autofluorescence characteristics, and emitted lightranged from 530 -600 nm at the condition of 488 nm light excitation, which distributed evenly with clear major shape. Autofluore scence of the two teliospores showed significant difference in space distribution by layer-cut scanning. Autofluore scencesubstances in TCK teliospores mainly distributed on outer spore wall and net ridge, but less in protoplasm. Autofluorescence substances in TFL teliospores mainly distributed in spore wall, but less in protoplasm.

关 键 词：激光共聚焦显微技术 小麦矮腥黑穗病病菌 小麦光腥黑穗病病菌 自发荧光特性 

分 类 号：S41-31[农业科学—植物保护]