土槿皮乙酸抗肝癌机制研究  

Anti-tumor Effect of Pseudolaric Acid B on Hepatocellular Carcinoma Hep G2 Cell Line

作　　者：钟婷[1] 薛变变 王增艳[3] 王莹[2] 刘春禹[4] 于静华[5] 胡冬华[1] 

机构地区：[1]长春中医药大学药学院药物化学教研室,吉林长春130000 [2]吉林大学白求恩第一医院胃肠内科,吉林长春130000 [3]吉林大学白求恩第一医院呼吸内科,吉林长春130000 [4]长春中医药大学附属医院针灸科,吉林长春130000 [5]吉林大学白求恩第一医院艾滋病与病毒研究所,吉林长春130000

出　　处：《中国现代中药》2016年第2期151-155,共5页Modern Chinese Medicine

基　　金：国家自然科学基金(81301416);吉林省科技厅重点科技攻关项目(20140204004YY);国家博士后基金(2014M561302)

摘　　要：目的:在细胞水平研究土槿皮乙酸(PAB)抗人肝癌细胞(Hep G2)的机制。方法:以4μmol·L-1PAB分别处理12、24、36、48 h后,通过细胞计数法确定PAB的抑制率;通过细胞形态学观察确定PAB是否诱导细胞死亡;划痕实验观察PAB对肝癌细胞转移的影响;荧光染色法检测PAB处理后Hep G2细胞中微管聚集情况;Realtime PCR检测PAB对P53基因转录的影响;Western blot检测相关蛋白表达。结果:PAB时间依赖性地抑制Hep G2细胞的生长,并且诱导Hep G2细胞的死亡;有效降低肝癌细胞的转移;促进细胞骨架蛋白聚集;降低P53基因转录,并调控P53蛋白和P27蛋白表达。结论:PAB对体外培养的Hep G2细胞生长有明显的抑制作用,并且通过调控微管分布来抑制细胞转移。Objective： The aim of this study was to investigate the anti-tumour role of pseudolaric acid B（ PAB） on hepatocellular carcinoma Hep G2. Methods： Cell counting analysis confirmed the inhibitory ratio of PAB. The morphological changes were observed by phase contrast microscopy. Scratch test was applied for cell immigration. Phalloidin –Tetramethylrhodamine B isothiocyanate staining was performed for tubulin polymerization. Real time PCR detected the p53 transcription. Western blot was used for protein expression. Results： The inhibitory role of PAB was in time dependent manner. PAB treatment induced the cell death. Cell immigration was inhibited by PAB treatment. Intervening α-tubulin polymerization was visualized after PAB treatment. Real-time PCR test confirmed that PAB treatment inhibited the transcription of p53 gene. And western blot analysis found that PAB regulated the expression of P53 and P27 protein. Conclusion： Therefore PAB had anti-tumor effect on Hep G2 through various mechanisms.

关 键 词：土槿皮乙酸 人肝癌Hep G2细胞 细胞增殖 细胞转移 

分 类 号：R965[医药卫生—药理学]