岗梅根、茎体外抗呼吸道病毒的作用比较研究  

Comparative Study on Effect of Ilex asprella Root and Stem against Respiratory Viruses

作　　者：陈炜璇[1,2] 韩正洲[1,2] 仰铁锤 马庆 詹若挺[2] 谈英 陈蔚文[2] 张奉学[3] 

机构地区：[1]华润三九医药股份有限公司,广东深圳518110 [2]广州中医药大学中药资源科学与工程研究中心岭南中药资源教育部重点实验室,广东广州510006 [3]广州中医药大学热带医学研究所,广东广州510405

出　　处：《中国现代中药》2016年第2期156-160,163,共6页Modern Chinese Medicine

基　　金："十二五"科技重大专项(2012ZX09201-201-001);广东省科技计划项目(2012A030100006)

摘　　要：目的:观察比较岗梅根和岗梅茎水提物对多种呼吸道病毒的抑制作用,为岗梅药材扩大药用部位提供参考。方法:分别接种甲1型流感病毒FM1株、呼吸道合胞病毒、鼻病毒R14型、腺病毒3型和副流感病毒3型于MDCK、A549、HEL和LLC-MK2细胞,采用鸡红血球凝集法观察岗梅根、茎水提物对甲1型流感病毒FM1株的抑制作用,采用细胞病变抑制法(CPE)观察岗梅根、茎水提物对呼吸道合胞病毒、鼻病毒R14型、腺病毒3型和副流感病毒3型的抑制作用。结果:在无毒浓度下,岗梅根水提物能抑制甲1型流感病毒FM1株、腺病毒3型、合胞病毒RSV和副流感3型病毒,而岗梅茎水提物仅能抑制腺病毒3型及呼吸道合胞病毒RSV。结论:岗梅根、茎水提物均有一定的体外抗呼吸道病毒作用,岗梅根水提物体外对呼吸道病毒的抑制作用略优于岗梅茎。Objective： To observe and compare the inhibitory effect of Ilex asprella root aqueous extract and I. asprella stem aqueous extract on several respiroviruses, in order to provide references for expansion of I. asprella＇s medicinal parts. Methods： MDCK,A549, HEL and LLC-MK2 cell were infected with influenza virus type A FM1 strain, RSV,rhinovirus type 14,adenovirus type 3,parainfluenza virus type 3 separately. The inhibitory effect of I. asprella root aqueous extract and I. asprella stem aqueous extract on influenza virus type A FM1 strain was observed by using chicken hemagglutination method. And CPE was used to observe the inhibitory effect of I. asprella root aqueous extract and I. asprella stem aqueous extract on RSV,rhinovirus type 14,adenovirus type 3,parainfluenza virus type 3. Results： At the nontoxic concentration,I. asprella root aqueous extract can inhibit influenza virus type A FM1 strain,RSV and parainfluenza virus type3,while I. asprella stem aqueous extract only inhibit adenovirus type 3 and RSV. Conclusion： Both I. asprella root aqueous extract and I. asprella stem aqueous extract can inhibit some of respiratory viruses in vitro. The inhibitory effect of I. asprella root aqueous extract on respiratory viruses in vitro is better than that of I. asprella stem aqueous extract.

关 键 词：岗梅根 岗梅茎 体外抗病毒 呼吸道病毒 

分 类 号：R725.6[医药卫生—儿科]