海藻酸钠-聚丙烯酰胺固定氧化亚铁硫杆菌技术的研究  

Technology of immobilized acidithiobacillus ferrooxidans with SA-HPAM

作　　者：廖泽君[1] 蔡萍莉[2] 王永东 史文革[1] 邹延[1] 

机构地区：[1]南华大学核资源工程学院,湖南衡阳421001 [2]南华大学城市建设学院,湖南衡阳421001 [3]铀矿冶生物技术国防重点学科实验室,湖南衡阳421001

出　　处：《中国矿业》2016年第3期150-153,157,共5页China Mining Magazine

摘　　要：以海藻酸钠(SA)、聚丙烯酰胺(HPAM)为复合载体,加入聚乙二醇(PEG)为致孔剂,对氧化亚铁硫杆菌进行固定化研究。实验考察了SA、HPAM、PEG的浓度对SA-HPAM凝胶微球的机械强度及固定化细胞的氧化活性的影响,确定了三种材料的最佳浓度:SA为2.5%,HPAM为0.4%,PEG为2%,此条件下固定化细胞对Fe^(2+)的最大氧化速率可达2.853g·(L·h)^(-1),24h后Fe^(2+)的氧化率在98%以上,固定化细胞目前重复使用了15次,并在3℃冰箱中存放两个月后,依然具有很好的氧化活性,表明SAHPAM固定化的氧化亚铁硫杆菌具有较好的稳定性。The SA and HPAM was used as compound carrier,and the PEG was added as pore-foaming agent to study the immobilized technology of acidithiobacillus ferrooxidans.Effect of concentration of the three materials on the mechanical strength of gelatinous microspheres and the activity of immobilized cells have been investigated.The optimal concentration of the three materials for SA-HPAM co-immobilization was obtained：mass fraction of SA solution 2.5%,mass fraction of HPAM solution 0.4%,volume fraction of PEG solution 2%.The rate of maximum oxidation was 2.853g·（L·h）^（-1） of the immobilized cells for Fe^（2＋）,and 98% of ferrous ions can be oxidized within 24 hours in that optimal condition.The oxidative activity of the immobilized cells still kept all right after the immobilized cells were reused for 15 times and refrigerated in the 3℃refrigerator for two months.It show the good stability of SA-HPAM co-immobilized Acidithiobacillus ferrooxidans.

关 键 词：固定化 氧化亚铁硫杆菌 海藻酸钠 聚丙烯酰胺 氧化 稳定性 

分 类 号：Q939.9[生物学—微生物学]