Isolation and Purification of Enzymatic Hydrolysates of Yam(Dioscorea opposita Thunb.)Proteins  

作　　者：MengchenXU Ke DING Ying LU Tao HAN 

机构地区：[1]Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue,Faculty of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206,China

出　　处：《Agricultural Biotechnology》2016年第1期19-21,24,共4页农业生物技术（英文版）

基　　金：Supported by Research Project of Development and Biological Activity of Functional(Health-care)Food Products(PXM2013_014207_000048);Processing and Storage of Agricultural Products-Beijing Key Construction Discipline(PXM2013-014207-000057)

摘　　要：Using yam （Dioscorea opposita Thunb. ） as the experimental material, enzymatic hydrolysates of yam proteins were prepared with alkaline protease, which were then isolated and purified by cellulose DE-52 anion exchange chromatography Sephadex G-50 gel chromatography. According to the results, four absorp- tion peaks were obtained by cellulose DE-52 anion exchange chromatography, and fractions at each absorption peak were collected. Specifically, the reducing ability of four peaks demonstrated a descending order of peak 2 （P2） 〉 peak I （ P1 ） 〉 peak 3 （P3） 〉 peak 4 （P4）. By Sephadex G-50 gel chmmatography, P1 and P2 were isolated and purified with distilled water and Tris-HC1 buffer, and two absorption peaks were obtained, respectively.Using yam （Dioscorea opposita Thunb. ） as the experimental material, enzymatic hydrolysates of yam proteins were prepared with alkaline protease, which were then isolated and purified by cellulose DE-52 anion exchange chromatography Sephadex G-50 gel chromatography. According to the results, four absorp- tion peaks were obtained by cellulose DE-52 anion exchange chromatography, and fractions at each absorption peak were collected. Specifically, the reducing ability of four peaks demonstrated a descending order of peak 2 （P2） 〉 peak I （ P1 ） 〉 peak 3 （P3） 〉 peak 4 （P4）. By Sephadex G-50 gel chmmatography, P1 and P2 were isolated and purified with distilled water and Tris-HC1 buffer, and two absorption peaks were obtained, respectively.

关 键 词：Yam proteins Enzymatic hydrolysis Isolation PURIFICATION 

分 类 号：Q946[生物学—植物学]