肿瘤坏死因子α与基质金属蛋白酶2及胶原在大鼠压疮皮肤组织中的表达及意义  

Expression and significance of tumor necrosis factor alpha, matrix metalloproteinase 2 and collagen in skin tissue of pressure ulcer of rats

作　　者：王晓慧[1] 毛挺挺 潘莹莹[2] 谢浩煌[1] 张宏宇[2] 肖健[2] 姜丽萍[1] 

机构地区：[1]浙江温州医科大学护理学院,325035 [2]浙江温州医科大学药学院,325035

出　　处：《中华烧伤杂志》2016年第3期160-167,共8页Chinese Journal of Burns

基　　金：国家自然科学基金（81372064）

摘　　要：目的观察TNF-α、基质金属蛋白酶2（MMP-2）及胶原在大鼠压疮局部皮肤组织中的表达，探讨压疮形成的可能机制。方法将40只SD雄性大鼠按随机数字表法分为正常对照组、受压3d组、受压5d组、受压7d组、受压9d组，每组8只。正常对照组大鼠不做任何处理；后4组大鼠应用缺血一再灌注磁片循环压迫的方式，在大鼠两侧后肢股薄肌处建立深部组织损伤模型（受压3d组）和压疮模型（另3组）。受压3d组大鼠仅受压3个循环；受压5d组、受压7d组、受压9d组大鼠在受压3个循环后，划破受压皮肤，后继续分别施压至5、7、9个循环。受压3d组大鼠于受压3d后（正常对照组大鼠于相同时间点），另3组大鼠分别于受压5、7、9d后，切取两侧后肢股薄肌中心部位处的皮肤组织。HE染色观察皮肤组织形态，Masson染色观察胶原纤维表达，免疫组织化学法检测Ⅳ型胶原、MMP-2表达，蛋白质印迹法检测TNF-α、磷酸化NF—κB表达。对数据行单因素方差分析、LSD检验。结果（1）正常对照组大鼠皮肤组织为复层鳞状上皮，皮肤结构清晰，无明显炎性细胞浸润；受压3d组大鼠上皮层次清晰，Fh较多，炎性细胞开始浸润；受压5d组、受压7d组、受压9d组大鼠随着受压时间延长，表皮增厚，Fb数量减少，炎性细胞浸润增强。（2）正常对照组大鼠皮肤组织中胶原纤维排列整齐，含量丰富。受压3d组大鼠皮肤组织中胶原纤维排列有序，表达量仍较高，与正常对照组大鼠相近（P〉0．05）。受压5d组、受压7d组大鼠皮肤组织中胶原纤维排列紊乱，表达量逐渐减少，均明显低于正常对照组（P值均小于0．01）；受压9d组大鼠皮肤组织中胶原纤维表达量较受压7d组少量增加，但仍明显低于正常对照组（P〈0．01）。（3）正常对照组、受压3d组、受压5d组、受压7d组、受压9d组大鼠皮肤组织中Ⅳ型胶原�Objective To observe the expressions of tumor necrosis factor alpha （TNF-α） , matrix metalloproteinase 2 （MMP-2） and collagen in local skin tissue of pressure ulcer of rats, and to explore the possible mechanism of the pathogenesis of pressure ulcer. Methods Forty male SD rats were divided into normal control group, 3 d compression group, 5 d compression group, 7 d compression group, and 9 d compression group according to the random number table, with 8 rats in each group. The rats in normal control group did not receive any treatment, whereas the rats in the latter 4 groups were established the deep tissue injury model （3 d compression group） and pressure ulcer model （the other 3 groups） on the gracilis muscle on both hind limbs using a way of cycle compression of ischemia-reperfusion magnet. The rats in 3 d com- pression group received only three cycles of compression, while the compressed skin of the rats in 5 d com- pression group, 7 d compression group, and 9 d compression group were cut through and received pressure to 5, 7 and 9 cycles after three cycles of compression, respectively. The rats in 3 d compression group were sacrificed immediately after receiving compression for 3 d （ the rats in normal control group were sacrificed at the same time） , and the rats in the other 3 groups were respectively sacrificed after receiving compression for 5, 7, and 9 d, and the skin tissue on the central part of gracilis muscle on both hind limbs were harvested. The morphology of the skin tissue was observed with HE staining. The expression of collagen fiber was ob- served with Masson staining. The expressions of collagen type Ⅳ and MMP-2 were detected by immunohisto- chemical method. The expressions of TNF-α and phosphorylated NF kappa B （NF-κB） were determined by Western blotting. Data were processed with one-way analysis of variance and LSD test. Results （1） In normal control group, the skin tissue of rats was stratified squamous epithelium, with the clear skin structure, and there

关 键 词：压力性溃疡 肿瘤坏死因子A 基质金属蛋白酶2 胶原Ⅳ型 

分 类 号：R473.6[医药卫生—护理学]