姜黄素-汉防己碱共递送抗病毒复方脂质体的构建  

Preparation of curcumin-tetrandrine co-delivery antiviral compound recipe liposome

作　　者：孙京芳 吉美芳 徐正行 刘宗亮[1] 赵烽[1] 陈大全[1] 

机构地区：[1]烟台大学分子药理和药物评价教育部重点实验室、新型制剂与生物技术药物研究山东省高校协同创新中心、长效和靶向制剂国家重点实验室,山东烟台264005 [2]青岛大学附属医院,山东青岛266003 [3]烟台市食品药品检验所,山东烟台264000

出　　处：《药学研究》2016年第3期157-160,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金项目(No.81302718、No.81573614);烟台市科技发展计划项目(No.2015ZH079)

摘　　要：目的制备寡聚透明质酸衍生物oHA修饰的姜黄素-汉防己碱中药抗病毒脂质体,并对其进行体外释放和稳定性研究。方法用薄膜分散法制备了寡聚透明质酸衍生物修饰的姜黄素-汉防己碱脂质体,以粒径和包封率作为两个重要的参考指标,使用粒度仪测定了粒径,使用紫外分光光度法测定包封率。结果用Box-Behnken效应面优化法确定了最佳磷脂胆固醇比为2.5∶1,最佳姜黄素-汉防己碱比例为2.8∶1,寡聚透明质酸衍生物的用量为1.80%,通过最佳处方制备的脂质体的粒径是201.06 nm,包封率是70.94%。结论制备的脂质体具有良好的稳定性,均匀的粒径分布,证明了脂质体具有良好的体外释放活性,良好的稳定性,为进一步研究抗病毒联合机制奠定基础。Objective To prepare hyaluronan oligosaccharides(oHA)modified antiviral liposome containing curcumin and tetrandrine,and study its release in vitro and stability.Methods The co -deliver antiviral liposomes was prepared by film dispersion method with particle size and entrapment rate as two important indicators,using particle size analyzer to de-termine the particle size and ultraviolet spectrophotometric method for the determination of entrapment efficiency.Results With Box - Behnken response surface optimization method,the optimal preparation (cholesterol and phospholipid ratio) was 2.5∶1.The best curcumin -tetrandrine proportion was 2.8∶1,and oHA was 1.80%.The particle size was 201.06 nm and entrapment efficiency was 70.94%.Conclusion The preparation method showed that liposomes had good stability,u-niform particle size distribution.It proved that the liposome had good in vitro release activity,good stability and had the po-tential use for antiviral nanocarriers.

关 键 词：姜黄素 汉防己碱 抗病毒 脂质体 共递送 

分 类 号：R944.9[医药卫生—药剂学]