异氟烷对人精子运动功能和顶体反应影响研究  

General anesthetic effects on human sperm motility function and acrosome reaction

作　　者：刘庆德[1] 王志丰[1] 虞利民[2] 

机构地区：[1]绍兴第二医院麻醉科,浙江绍兴312000 [2]绍兴第二医院泌尿外科,浙江绍兴312000

出　　处：《中国性科学》2016年第2期105-107,共3页Chinese Journal of Human Sexuality

摘　　要：目的:观察异氟烷对人精子运动功能、体外获能以及精子顶体反应的影响,对有生育要求的男性提供临床用药依据。方法:收集我院男科门诊就诊的男性精子标本,从中筛选出18份活力正常精子的精液作为标本,每例精液均分成3份,分别放置于3个试管中,分别给予1.1%异氟烷(ISO1组)、2.2%异氟烷(ISO2组)和空气作为对照组,把所有的有机玻璃箱放置于孵箱培养5h,对精子的运动、获能、顶体反应进行评价。结果:1.1%和2.2%浓度的异氟烷作用于精子5h以后,与对照组相比,精子的运动活力、直线速度、曲线速度、平均速度均明显下降,P<0.05,差异有统计学意义,ISO1组和ISO2组比较,ISO2组的下降更为明显,呈现剂量依赖关系,即随着浓度的升高,精子的运动功能抑制越明显。对于精子获能的影响,与对照组比较,两种浓度的异氟烷均明显地抑制精子的体外获能,P均<0.05,差异均有统计学意义,ISO1组和ISO2组比较无明显差异,P>0.05。两组精子发生顶体反应的百分率均较处理前明显增加,P<0.05,差异有统计学意义。但是处理后两组间比较无明显差异。结论:异氟烷能够抑制精子的运动和获能,并且随着浓度的增加,抑制作用增强;异氟烷对精子顶体反应影响不明显。Objectives： To observe the effect of isoflurane general anesthetic on sperm movement function,in vitro capacitation and sperm acrosome reaction,so as to provide basis for clinical application. Methods： The sifted 18 specimens of normal sperm from male sperm samples in male hospital outpatient clinic were selected and each sperm sample was divided into three parts. In three test tubes,respectively,they were given 1. 1% isoflurane（ ISO1）,2. 2% isoflurane（ ISO2） and air as the control group. All the organic glass boxes were placed in incubator for 5 h cultivation to evaluate sperm movement and acrosome reaction. Results： After 5 h of 1. 1% and 2. 2%isoflurane treated on sperm,compared with control group,the sperm vitality,linear velocity,the curve speed and average speed significantly decreased,with statistically significant difference（ P〈0. 05）. Compared with ISO1 and ISO2 group,ISO2 group decreased more obviously,which presented a dose- response relationship. Namely with the increase of concentration,the inhibition effect on sperm motion function was more obvious. For the influence of capacitation,compared with control group,both kinds of concentrations of isoflurane could obviously inhibit in vitro capacitation,with statistically significant difference（ P〈0. 05）. ISO1 and ISO2 group had no significant difference,P〉0. 05. The percentages of the sperm acrosome reaction of the two groups were also significantly increased before treatment,with statistically significant difference（ P〈0. 05）. But there was no significant difference between the two groups after treatment. Conclusion： Isoflurane can inhibit sperm movement and capacity. With the increase of concentration,the inhibition increases and Isoflurane has no obvious effect on sperm body reaction.

关 键 词：麻醉 异氟烷 精子运动 精子获能 顶体反应 

分 类 号：R917.2[医药卫生—药物分析学]