基于转录组测序技术的重症手足口病相关基因研究  

Research of the severe hand, foot and mouth diseases related susceptibility genes based on the transcriptome sequencing technologies

作　　者：陈程[1] 刘映霞[2] 杨桂林[2] 邓永[2] 单灵波 邹容容[2] 彭忠田[1] 

机构地区：[1]南华大学附属第一医院感染科,衡阳市421001 [2]南华大学附属深圳市第三人民医院感染科,深圳市518112

出　　处：《中华实验和临床感染病杂志（电子版）》2016年第1期26-30,共5页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：深圳市新发传染病重点专科基金(N o.201161);深圳市科技创新委项目(N o.JCYJ20150402111430623)

摘　　要：目的应用转录组测序技术(RNA-seq)分析重症手足口病(SHFMD)患者外周血单个核细胞(PBMC)转录组情况,筛选SHFMD相关基因,探讨其发生的可能免疫机制。方法收集2014年5月至8月深圳市第三人民医院收治的重症和轻症手足口病(MHFMD)患儿各5例,以及5例健康体检儿童的外周血,均分离PBMC,采用转录组测序技术筛选显著的差异表达基因(DEGs),并通过实时荧光定量PCR对DEGs进行验证。结果 SHFMD组相对健康对照组共117个DEGs,其中108个基因上调,9个基因下调;SHFMD组相对MHFMD组共26个DEGs,其中14个基因上调,12个基因下调;两组DEGs中共有8个相同基因,其中TNFRSF13C、IL-7R、THBS1和VEGFA下调,S100A8、S100A12、IL-8和IL-1β上调(P均<0.05)。结论 S100A8、S100A12、IL-8、IL-1β、TNFRSF13C、IL-7R、THBS1和VEGFA是SHFMD相关的差异表达基因,可能在SHFMD的发生中起重要作用,有望成为手足口病重症化的预测基因。Objective To screen severe hand, foot and mouth disease（SHFMD） related genes, and to explore the possible immune mechanisms by high-throughput transcriptome sequencing technology（RNAseq）. Methods Total of 15 PBMC samples were collected from 5 patients with SHFMD, 5 patients with mild hand, foot and mouth disease（MHFMD） and 5 cases of healthy control（HC）, during May 2014 and August 2014, in the Third People＇s Hospital of Shenzhen. The RNA of the PBMCs were sequenced by the RNA-seq technology, on the basis of RPKM（reads per kilobase of exon model per million mapped reads） multiples and expression level fold-change, and the differentially expressed genes（DEGs） were screened. Furthermore, the expression levels of the DEGs by quantitative real-time PCR were confirmed. Results Total of 117 DEGs between SHFMD and MHFMD were found, including 108 up-regulated genes and 9 down-regulated genes; and 26 DEGs between SHFMD and HC, including 14 up-regulated genes and 12 down-regulated genes. The two groups of DEGs had 8 genes in common, among which S100A8, S100A12, IL-8, IL-1β were upregulated, while TNFRSF13 C, IL-7R, THBS1,VEGFA were down-regulated（P〈0.05）. ConclusionsS100A8, S100A12, IL-8, IL-1β, TNFRSF13 C, IL-7R, THBS1 and VEGFA are SHFMD specific DEGs, which may play an important role in the development of SHFMD and potentially serve as gene markers to predict the occurrence of SHFMD.

关 键 词：转录组测序 重症手足口病 预测基因 

分 类 号：R725.1[医药卫生—儿科]