基于直立碳纳米管大面积金粒子的DNA生物传感器用于早幼粒白血病/维甲酸受体α融合基因检测  

DNA Biosensor Based on Large Area of Gold Particles on Aligned Carbon Nanotubes for Detection of Promyelocytic Leukaemia / Retinoic Acid Receptor α Fusion Gene

作　　者：杨丽珠[1] 朱婧[2] 章仁毅[2] 马丹琦 何品刚[2] 方禹之[2] 

机构地区：[1]温州医科大学药学院,温州325035 [2]华东师范大学化学系,上海200241 [3]浙江省台州市路桥区计划生育宣传技术指导站,台州318050

出　　处：《分析化学》2016年第3期391-395,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.31300819);浙江省自然科学基金项目(No.LQ12B05004)资助~~

摘　　要：基于直立碳纳米管上的大面积金粒子构建了新型的电化学DNA生物传感器,用于急性早幼粒细胞白血病PML/RARα融合基因的检测。首先在直立碳纳米管电极表面溅射金粒子,采用自组装方法将巯基修饰的单链DNA固定到电极上,将氨基修饰的单链DNA和羧基化的Cd Te量子点通过酰胺缩合反应生成Cd Te修饰的DNA探针,通过与目标DNA的双杂交反应形成三明治结构,利用差分脉冲阳极溶出伏安法检测电极表面捕获的Cd Te量子点,从而对DNA进行定量分析。结果表明,电极上Cd^(2+)峰电流与目标DNA浓度(1.0×10^(-12)~1.0×10^(-8)mol/L)的对数值呈线性关系,线性方程为i_(pa)(μA)=1.626+0.132lg C(mol/L)(R=0.996),检出限为4.0×10^(-13)mol/L(3σ)。传感器表现出良好的重现性和稳定性。A novel electrochemical DNA biosensor was fabricated based on the large area of gold particles on the aligned carbon nanotubes for the detection of promyelocytic leukaemia / retinoic acid receptor α（ PML /RARα） fusion gene in acute promyelocytic leukemia. Firstly, the thiol-modified single-stranded DNA（ ss DNA,probe 1） was immobilized on the gold particles sputtered on the aligned carbon nanotubes electrode by self-assembly technique. Then,amino-modified ss DNA and carboxyl-modified Cd Te quantum dots（ QDs）were combined to get Cd Te-modified DNA probe through an amidization reaction. After hybridization with target DNA,a sandwich-type assay structure was formed. Finally,the Cd Te quantum dots captured on the electrode surface were detected by differential pulse anodic stripping voltammetry. The change of peak current of Cd^（2＋）on the electrode was found to be linear with the logarithm of target DNA concentration in the range of1. 0 ×10^（-12）mol / L to 1. 0 ×10^（-8）mol / L. The Linear equation was i（pa）（ μA） = 1. 626＋0. 132 lg C（ mol/L）（ the correlation coefficient R was 0. 996） and the detection limit was 4. 0 ×10^（-13）mol / L（ 3σ）. The fabricated DNA biosensor exhibited excellent reproducibility and stability.

关 键 词：直立碳纳米管 金粒子 急性早幼粒细胞白血病 DNA生物传感器 差分脉冲阳极溶出伏安 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]