牛场肥水灌溉对土壤反硝化细菌nosZ基因组成结构和多样性研究  

Effects of Cattle Fertilizer on Community Structure and Diversity of nosZ-type Denitrifying Bacteria in Soil

作　　者：王婷[1,2] 刘丽丽[1] 高文萱[2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]农业部环境保护科研监测所,天津300191

出　　处：《天津农业科学》2016年第3期11-16,共6页Tianjin Agricultural Sciences

基　　金：国家自然科学基金面上项目(41371481);国家科技支撑计划(2012BAD15B02)

摘　　要：以河北省徐水县长期定位施肥试验田为研究对象,利用末端限制性片段长度多态性(T-RFLP)分析和克隆文库构建,研究了5种施肥处理(清水灌溉CK、无机肥灌溉CF、不同牛场肥水灌溉T4、T5和T11)下土壤中nos Z型反硝化细菌群落多样性及其群落结构演变。结果表明,不同施肥处理下nos Z型反硝化细菌群落多样性无显著性差异,施肥条件对nos Z型反硝化细菌的群落结构无明显影响,说明本试验土壤中nos Z型反硝化细菌对施肥不敏感,群落稳定。系统发育分析结果表明,nos Z型反硝化细菌主要与假单胞菌属(Pseudomonas)具有较近的亲缘关系。The community structure and diversity of nosZ-type denitrifying bacteria under various fertilization regimes including control without fertilizers （CK）, conventional fertilization（CF）and cattle fertilizer irrigation treatments in three fertilizer levels（T4, T5, T11） in Xushui, Hebei Province were investigated by the combination of terminal restriction fragment length polymorphism（T-RFLP） analysis and gene sequencing. The results showed that no significantly difference was noted in nosZ-type denitrifying bacteria diversity indexs in all treatments, and fertilization regimes have no significantly influenced on community composition of nosZ-type denitrifying bacteria, indicating that nosZ-type denitrifying bacteria have a stable community structure in 0-5 cm soil of this study. Phylogenetic analysis indicated that the Alcaligene, Rhizobium and nosZ-type denitrifiers were mainly composed of Pseudomonas.

关 键 词：NOSZ 反硝化细菌 牛场肥水灌溉 T—RFLP 群落多样性 

分 类 号：S155.44[农业科学—土壤学]