米氏硫胺素芽孢杆菌的分离及鉴定  

Isolation and Identification of Aneurinibacillus migulanus

作　　者：张晶[1] 徐宏伟[1] 曾国航 宋显明[1] 艾东旭 李莲瑞[2,3] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木大学动物科学学院,新疆阿拉尔843300 [3]塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300

出　　处：《新疆农业科学》2016年第1期59-67,共9页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30690277);兵团应用基础项目(2007JC07)~~

摘　　要：【目的】分离、纯化土壤中的细菌,通过鉴定得到米氏硫胺素芽孢杆菌。【方法】通过生化鉴定、提取细菌基因组并进行细菌16 s rDNA测序等方法鉴定。【结果】此细菌生化鉴定结果符合《常见细菌鉴定手册》中的关于米氏硫胺素芽孢杆菌的生化鉴定,将细菌的16 s rDNA测序结果在NCBI上进行比对,表明该细菌为米氏硫胺素芽孢杆菌。【结论】分离、纯化得到的细菌经测序对比后确定是米氏硫胺素芽孢杆菌。【Objective】To isolate and purify bacteria in soil in the hope of obtaining Aneurinibacillus migulanus through the process of identification. 【Method】By biochemical identification,extracting bacterial genome,sequencing 16 s rDNA of bacterial methods to identify the bacteria. 【Result】This bacterial biochemical identification results were consistent with theoretical results of ＂ berger bacteria identification manual＂ about biochemical identification of Aneurinibacillus migulanus,and then bacterial 16 s rDNA sequencing results were compared on the NCBI. 【Conclusion】Bacteria isolated and purified were determined to be Aneurinibacillus migulanus by comparison of the sequenced bacillus.

关 键 词：细菌分离 细菌鉴定 16 s RDNA 米氏硫胺素芽孢杆菌 

分 类 号：S188[农业科学—农业基础科学]