限制性抗原亲和力与BED捕获酶免法用于HIV-1新发感染检测的比较研究  

A comparative study on using limiting antigen avidity enzyme immunoassay and BED capture enzyme immunoassay for the detection of HIV-1 new infection

作　　者：夏燕[1] 潘晓红[1] 徐云[1] 丁晓贝[1] 张佳峰[1] 张文君[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《中国卫生检验杂志》2016年第5期609-612,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家科技重大专项(2012ZX10001001);浙江省医药卫生科技计划(2014KYA035)

摘　　要：目的比较限制性抗原亲和力酶免法(简称LAg)与BED捕获酶免法(简称BED)用于人类免疫缺陷病毒1型(HIV-1)新发感染检测的结果,评价2种方法的适用性。方法应用LAg和BED 2种方法同时检测HIV抗体阳性急性感染期、发病期、常规报告病例样品,用Mc Nermar检验2种方法新发感染检出率和一致性。结果 24份HIV抗体阳性急性感染期病例样品,LAg判定新发感染23份(95.83%),BED判定新发感染24份(100.00%)。328份常规报告病例样品,LAg判定长期感染237份(72.26%),BED判定长期感染224份(68.30%)。71份发病期病例样品,LAg判定长期感染49份(69.01%),BED判定长期感染46份(64.79%)。Mc Nermar检验2种方法对常规报告病例、发病期病例样品及不同的CD4+T细胞计数判定新发感染结果一致性差异均有统计学意义(P<0.01)。结论 LAg新发感染检出率及错判率低于BED,受CD4+T细胞计数影响较BED小,更加真实地反映新发感染情况。Objective To compare the detection results with limiting antigen avidity enzyme immunoassay（ Lag） and BED capture enzyme immunoassay（ BED） in the new infection detection in human immunodeficiency virus type 1（ HIV-1）,and to assay the application of the two methods. Methods LAg and BED were applied separately in the samples which were at acute infection period,AIDS period and samples with routine case report. The detection rate and the consistency of the new detection results of the two methods were tested by Mc Nermar. Results LAg determined 23 samples as new infections（ 95. 83%） in 24 samples which were at acute infection period,while BED determined all of them（ 100. 00%）. LAg determined 237 samples（ 72. 26%） as long-term infections in 328 samples with routine case report,while BED determined 224 samples（ 68. 30%） as long-term infections. LAg determined 49 samples as long-term infections（ 69. 01%） in 71 samples which were at period of AIDS,while BED determined 46 samples（ 64. 79%） as long-term infections. There was statistical significance on the differences in the detection of new infection when applied in samples with different CD4＋T cell counts for the two method tested by Mc Nermar method（ P 0. 01）. Conclusion The detection rate of newly infection with LAg and false-positive rate was lower than that of BED,and which has less effect by CD4＋T cell count than that of BED,which can reflect the new infection situation more.

关 键 词：限制性抗原亲和力酶免法 BED捕获酶免法 HIV-1新发感染检测 研究 

分 类 号：R446.6[医药卫生—诊断学]