一种用于监控葡萄糖浓度的嵌入式光学生物传感器  

A Kind of Embedded Optical Biosensor Used to Monitor Glucose Concentration

作　　者：丁涛[1] 王芳[2] 

机构地区：[1]延安大学陕西省化学反应工程重点实验室,陕西延安716000 [2]延安大学石油学院,陕西延安716000

出　　处：《化学与生物工程》2016年第3期63-67,共5页Chemistry & Bioengineering

基　　金：陕西省教育厅自然科学专项(15JK1808)

摘　　要：由于在持续流动条件下连续监测血糖浓度较为困难,提出了2种硅橡胶（聚二甲基硅氧烷）光子生物传感器设计方法,分别是内部固定光子生物传感器和对内部固定光子生物传感器进行改进,将微珠腔与玻璃粉填充渠道综合的外部固定光子生物传感器。结果显示,前者内部固定光子生物传感器可检测浓度为0.26-5.00mg·L-1的葡萄糖,R2=0.9905;后者通过外部固定联合方法固定大量的酶于玻璃粉上,加快了传感器腔内化学反应的速率,在连续流动情况下,可检测浓度为0.7-10.0mg·L-1的葡萄糖,R2=0.9845。外部固定光子生物传感器在光子芯片实验室的在线分析方面具有广阔的应用前景。Since it is difficult for continuously monitoring blood sugar in a steady flow,two silicon rubber（PDMS）photonic biosensors were proposed in this paper.One is an internal fixed sensor and the other is a modified version of this sensor,i.e.,the beads cavity with glass powder filling composite in the sensor channels.Results showed that,the internal fixed sensor could detect glucose in the concentrations region of 0.26~5.00mg·L-1 with R2 of 0.9905.The external immobilization method offered the possibility of covalently immobilizing huge amount of enzymes to micro-beads,which promoted the rate of chemical reaction taking place inside the biosensor cavity.External fixed sensor could detect glucose in the concentrations region of 0.7-10.0mg·L-1 with R2 of 0.9845 under a continuous flow.The results have a good application prospect in photon system online analysis.

关 键 词：葡萄糖 酶 玻璃粉(微珠) 生物传感器 

分 类 号：O657.3[理学—分析化学]