1株甲基杆菌的鉴定  

Identification of a strain of Methylobacterium

作　　者：曹璟[1] 黄思佳[1] 郭蓉[1] 刘鹏[1] 金文平 胡业勤[1] 薛红刚[1] 

机构地区：[1]武汉生物制品研究所有限责任公司细菌类疫苗室,430207 [2]武汉市第三医院检验科,430060

出　　处：《国际生物制品学杂志》2016年第1期12-14,共3页International Journal of Biologicals

基　　金：“重大新药创制”科技重大专项（2013ZX09402-302）;武汉市关键技术攻关计划（2（）14060202010116）

摘　　要：目的鉴定1株在生物制品生产车间采集的菌株wh01的种属。方法观察该菌株的形态，并用细菌鉴定仪进行检定。采用PCR法扩增菌株的16SrDNA和甲醇脱氢酶大亚基的mxaF基因，用生物信息学软件进行分析。结果该菌株经细菌鉴定仪检定为沙门菌属，但其形态与沙门菌有很大差别，其16SrDNA和mxaF基因序列与甲基杆菌属序列的相似性高达99％。结论该菌株属于甲基杆菌属。Objective To identify a bacterial strain wh01 collected in a biological product workshop. Methods The strain was morphologically observed and identified by bacteria identification instrument. The 16S rDNA and methanol dehydrogenase large subunit mxaF gene were amplified by PCR and sequenced. Sequences were then analyzed by bioinformatics software. Results The strain was identified as a Salmonella sp. by the instrument, but morphologically different from Salmonella. The sequence similarity of its 16S rDNA and mxaF gene to that of Methylobacteriurn was as high as 99%. Conclusion This strain belongs to Methylobacteriurn genus.

关 键 词：甲基杆菌 DNA 核糖体 mxaF基因 环境污染 

分 类 号：R446.5[医药卫生—诊断学]