甲醛经口染毒致小鼠脏器损伤和炎症反应的研究  

Organ injure and inflammatory response of mice induced by oral exposure of formaldehyde

作　　者：赵云[1] 尤会会[1] 沈世萍 郭晴[1] 梅宇飞[1] 李金泉[1] 朱雨晴[1] 李潇潇[1] 丁书茂[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《中国环境科学》2016年第3期935-942,共8页China Environmental Science

基　　金：国家自然科学基金重点项目(51136002)

摘　　要：为探讨在单独染毒和卵清蛋白(OVA)联合致敏的条件下,甲醛灌胃染毒对小鼠造成的毒性效应,将雄性Balb/c小鼠随机分为7组:对照组(蒸馏水组);OVA致敏组;2mg/(kg?d)FA(甲醛)组;20mg/(kg?d)FA组;200mg/(kg?d)FA组;200mg/(kg?d)FA+OVA组;200mg/(kg?d)FA+OVA+MT(褪黑素)组,以蒸馏水和不同浓度甲醛溶液灌胃,连续21d.OVA致敏组,200mg/(kg?d)FA+OVA组,200mg/(kg?d)FA+OVA+MT组在第6,13,20d进行腹腔注射OVA致敏;此外,200mg/(kg?d)FA+OVA+MT组,每天用1.0mg/m L褪黑素灌胃小鼠(小鼠灌胃剂量10mg/(kg?d)),连续21d.检测肝,肾和肺组织中活性氧自由基(ROS),丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量,并采用ELISA法检测肝组织中IL-4和IFN-γ的水平.结果表明,与对照组相比,甲醛浓度为200mg/(kg?d),小鼠肝脏ROS含量增加(P<0.05),MDA含量增加以及小鼠肾脏GSH含量降低,均有显著性差异(P<0.05).OVA作为致敏剂,甲醛浓度为200mg/(kg?d)时,肝组织中IL-4(Interleukin-4)含量增加(P<0.01),10mg/(kg?d)褪黑素能够降低200mg/(kg?d)甲醛+OVA染毒小鼠肝脏内ROS含量(P<0.05).综上,200mg/(kg?d)甲醛灌胃染毒能使小鼠产生氧化损伤和炎症反应,ROS,MDA水平上升(P<0.05),GSH水平下降(P<0.05),肝脏中细胞因子IL-4水平上升(P<0.01),IFN-γ水平下降(P<0.05).To explore the effects on mice through administration of formaldehyde（FA） by gavage with or without ovalbumin（OVA）, which lead to the inflammation in body, the male Balb/c mice were randomly divided into 7groups： the control group（distilled water group）, OVA group, 2mg/（kg·d） FA（formaldehyde） group, 20mg/（kg·d） FA group, 200mg/（kg·d） FA group, 200mg/（kg·d） FA＋OVA group and 200mg/（kg·d）FA＋OVA＋ MT（melatonin） group. Mice were gavaged with distilled water and different concentrations of FA solution respectively for 21 days. The mice in OVA group, 200mg/（kg·d） FA＋OVA group and 200mg/（kg·d） FA＋OVA＋MT group were sensitized with OVA in day 6, 13, 20 by intraperitoneal injection, and the mice in 200mg/（kg·d） FA＋OVA＋MT group were also challenged with 1mg/m L MT by gavage for 21 days. The contents of ROS, MDA and GSH in liver, kidney and lung tissue homogenate were determined. IL-4and IFN-g concentrations in liver were measured by ELISA kits. The results showed that compared with control group, the livers ROS of mice in 200mg/（kg·d） FA group reached a significant level（P〈0.05）, and MDA increased significantly（P〈0.05）; GSH content in kidneys decreased significantly（P〈0.05）. IL-4 content in livers of mice in 200mg/（kg？d） FA＋OVA group significantly increased（P〈0.01）. In 200mg/（kg·d） FA＋OVA group, the administration of 10mg/（kg·d） MT can reduce ROS in the livers significantly（P〈0.05）. In a word, the gavage of 200mg/（kg·d） FA would induce oxidative damage and inflammation in mice. ROS, MDA in 200mg/（kg·d） FA group reached a significant level（P〈0.05）, on the contrary, GSH content decreased significantly（P〈0.05）. In 200mg/（kg·d） FA＋OVA group, IL-4 content in livers of mice significantly increased（P〈0.01）, while IFN-g content had no significant change（P〉0.05）.

关 键 词：甲醛 卵清蛋白 小鼠 氧化损伤 炎症反应 

分 类 号：X503.22[环境科学与工程—环境工程]