微波辅助萃取益母草中挥发油含量研究  

Study on Content of Volatile Oil in Leonurus japonicus Houtt.by Microwave-assisted Extraction

作　　者：孙玲 杨肖 李世民[2] 孙晓倩[3] 孙蓉[2] 

机构地区：[1]山东省药学科学院,山东济南250101 [2]山东省中医药研究院,山东济南250014 [3]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2016年第1期13-15,共3页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金项目(81450021);山东省自主创新和成果转化项目(2014ZZCX02104)

摘　　要：目的探讨微波辅助萃取益母草中挥发油的效果并确定最佳萃取条件。方法采用微波辅助萃取和水蒸气蒸馏两种方法提取益母草中的挥发油,优化了萃取条件:溶剂体积、萃取功率、萃取时间,并用气相色谱对其成份进行定性鉴定和相对含量的分析。结果微波辅助萃取法和水蒸气蒸馏法对益母草挥发油提取率分别为0.110%和0.099%。结论微波辅助萃取益中挥发油母草的提取效果优于水蒸气蒸馏法。Objective To discuss the extraction effect of volatile oil from Leonurusjaponicus Houtt. by microwave-assisted extraction and optimum extract parameters. Methods Two methods of hydro-distillation and microwave-assisted extraction were used for extracting volatile oil from Leonurus japonicus Houtt., then optimized the extraction conditions： solvent volume, extracting power, extracting time, and gas chromatography was used for the qualitative identification and relative content analysis of component. Results The extracting yields of volatile oil from Leonurus japonicus Houtt. by microwave-assisted extraction of and hydro-distillation were 0.110% and 0.099%, respectively. Conclusion The extraction effect of volatile oil from Leonurus japonicus Houtt. by microwave-assisted extraction was better than hydro-distillation.

关 键 词：益母草 挥发油 微波辅助萃取 

分 类 号：R917[医药卫生—药物分析学]