金铁锁去皮根、带皮根、根皮水煎液对小鼠急性毒性的实验研究  

Comparison Study on Acute Toxicity of the Aqueous Solution from Radix, Radix with Cortex and Cortex of Psammosilene Tunicoides

作　　者：吴玟萱 郭建友[2] 王谦[1] 张北月 王胜男[1] 马晓杰[1] 郑志全[1] 石晋丽[1] 

机构地区：[1]北京中医药大学,北京100102 [2]中国科学院心理研究所,北京100101

出　　处：《中国药物警戒》2016年第2期70-73,77,共5页Chinese Journal of Pharmacovigilance

基　　金：国家药典委员会"国家药品提高暨2015版药典科研任务"项目(金铁锁药材及饮片质量标准的修订)

摘　　要：目的测定比较金铁锁去皮根、带皮根、根皮三样品水煎液的急性毒性,为临床安全合理用药提供依据。方法采用经典的急性毒性实验方法 ,进行金铁锁三样品水煎液的小鼠急性毒性实验比较,记录死亡情况、毒性症状、体重变化、死亡小鼠高剂量进行组织病理观察,用Bliss法计算半数致死量(LD_(50))。结果金铁锁去皮根、带皮根、根皮水煎液的LD_(50)值(95%可信限)分别为:4.638 2(4.004 6~5.439 6)g·kg^(-1)、4.847 1(4.325 1~5.450 8)g·kg^(-1)、6.403 2(5.720 7~7.210 3)g·kg^(-1)(生药量),毒性靶器官主要为肺、脾、胃。结论金铁锁不同部位水煎液的LD_(50)值:根皮>带皮根>去皮根,差异较小。其毒性体内过程、毒性机制等尚待进一步研究。Objective To compare the acute toxicity of the aqueous solution from Psammosilenes radix, Psammosilenes radix with cortex and Psammosilenes cortex, in order to provide a basis for safe and reasonable medication in clinic. Methods Three samples of Psammosilenes tunicoides were prepared and compared in accordance with classical acute toxicity test. The toxic symptom was observed, and the death and the change of body weight were recorded. The pathological morphology parameters of dead mice with high dose were performed. The experimental data are calculated LD_（50） with the Bliss method. Results The LD_（50）（the 95% confidence interval） of Psammosilenes radix, Psammosilenes radix with cortex, Psammosilenes cortex are respectively 4.638 2（4.004 6~5.439 6） g·kg^（-1）, 4.847 1（4.325 1~5.450 8） g·kg^（-1）, 6.403 2（5.720 7~7.210 3） g·kg^（-1）（crude drug dose）. The main toxic target organs are lung, spleen and stomach. Conclusion The value of LD_（50） of different fractions in Psammosilenes tunicoides is： Psammosilenes cortex 〉Psammosilenes radix with cortex 〉Psammosilenes radix. However, it has slight difference. The process of toxicity in vivo and toxicity mechanism need to be further studied.

关 键 词：金铁锁 水煎液 急性毒性 

分 类 号：R992[医药卫生—毒理学] R282[医药卫生—药学]