苦参碱镇痛作用及其阿片受体非相关性的研究  

Study on analgesic effect of matrine and non-correlation to opioid receptors

作　　者：杨丽[1] 邓扬鸥[2] 吴世星 吕晓强[1] 刘永刚[3] 

机构地区：[1]辽宁中医药大学基础医学院药理教研室辽宁中医药大学省部共建中医脏象理论及应用教育部重点实验室,沈阳110847 [2]中国医科大学附属第一医院干诊科,沈阳110001 [3]武警广东省总队医院药剂科,广州510507

出　　处：《中华中医药杂志》2016年第3期998-1001,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81102901);教育部留学回国人员科研启动基金项目(No.教外司留[2012]940号);辽宁省自然科学基金项目(No.201102151)~~

摘　　要：目的:探讨苦参碱(MT)对小鼠不同疼痛模型的镇痛作用及其与阿片受体的非相关性。方法:C57BL/6小鼠随机分为空白对照组、阳性对照组、纳洛酮组及给药组。采用醋酸扭体法,观察给药后小鼠扭体次数,探讨MT对化学刺激性疼痛的抑制作用;采用热板法,测定舔后足潜伏期,探讨MT对物理性疼痛的镇痛作用;并同时尾静脉注射阿片受体拮抗剂纳洛酮,观察其对MT镇痛作用的影响;采用部分坐骨神经结扎法(PSNL)建立神经性疼痛模型,用up and down方法分别测PSNL结扎后第7天小鼠给药前后手术侧后足足底机械缩足反应阈值,计算ED50,探讨MT(50.00mg/kg)对神经性疼痛的镇痛作用。结果:MT的镇痛作用在5-100mg/kg范围内呈良好的量效关系,MT25.00mg/kg剂量及以上能减少醋酸所致的扭体次数,50.00mg/kg及以上剂量MT能提高热刺激引起的痛阈(P<0.01),且以上镇痛作用不能被纳洛酮所拮抗;MT(50.00mg/kg)还可显著提高PSNL小鼠的机械缩足反应阈值ED50。结论:MT对物理性、化学性及神经性损伤性刺激均有镇痛作用,并且此镇痛作用并不依赖于激动阿片受体。Objective： To investigate analgesic effect of matrine（MT） on different pain models and the non-correlation between analgesic effect of matrine and opioid receptors. Methods： C57BL/6 mice were randomly divided into control group, morphine group, naloxone group and treatment group. Torsion times were observed by acetic acid writhing method, in order to evaluate the inhibition of matrine on chemical pain. Latent period of licking metapodium was measured by hot plate test, in order to determine the analgesic effect of matrine on physical pain. Meanwhile, tail intravenous injection of naloxone which was an opioid receptor antagonist was used to estimate the relation between analgesic effect of matrine and opioid receptors. Neuropathic pain model was established by using partial sciatic nerve ligation（PSNL）, up-and-down method was used to measure the mechanical withdraw threshold of operated pelma before and after treating on the 7th day after PSNL ligaturing, and the ED50 was calculated, in order to investigate the analgesic effect of matrine（50mg/kg） on neuropathic pain. Results： The analgesic effect of matrine had a good dose-response relationship within the scope of 5-100mg/kg. Matrine could reduce the writhing times induced by acetic acid（P0.01）, increased threshold of pain induced by hot-plate（P0.01）, and the above analgesic effect could not be antagonized by naloxone. Matrine（50mg/kg） also could increase the ED50 of mechanical withdraw threshold of PSNL mice. Conclusion： Matrine has analgesic effect on physical, chemical and neuropathic pain models, and the analgesic effect is not depended on stimulation of opioid receptors.

关 键 词：苦参碱 镇痛 疼痛模型 纳洛酮 阿片受体 

分 类 号：R285.5[医药卫生—中药学]