流感病毒H1N1感染A549细胞诱导炎性因子表达及黄芩苷调控作用的研究  

Expression of inflammatory cytokines in A549 cells infected by H1N1 and regulating effect of baicalin

作　　者：刘晓婷[1] 张沂[1] 顾立刚[1] 葛世杰[1] 吴珺[1] 邱泽计[1] 于卓男[1] 王玥琦[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2016年第3期1032-1035,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173371)~~

摘　　要：目的:探讨流感病毒H1N1体外感染细胞诱导炎性因子表达及黄芩苷调控作用的研究。方法:正常生长细胞接种100 TCID50的病毒液;吸附2h后加入黄芩苷高剂量组(3.96μg/m L)和黄芩苷低剂量组(0.99μg/m L),以奥司他韦(0.75μg/m L)做阳性对照。采用基因芯片技术分析各组细胞差异表达炎性因子。以RT-PCR和Western blotting法检测细胞内靶因子m RNA和蛋白的表达变化。结果:与细胞对照组相比,H1N1感染组差异表达基因IL-1β、IL-6、IL-8、TNF-α和RANTES明显上调(P<0.01);给药后上述因子表达下调,部分IL-10明显上调(P<0.05,P<0.01)。Western blotting和RT-PCR检验结果与基因芯片结果相符合。结论:流感病毒H1N1感染后诱导炎性因子过度表达损伤细胞,黄芩苷可以调控炎性因子表达,减轻宿主细胞的损伤作用,具有明显的抗感染在用。Objective： To investigate the expression of inflammatory cytokines in human pulmonary adenocarcinoma cell A549 infected by H1N1 in vitro and the regulating effect of baicalin. Methods： A549 cells were inoculated into culture solution contained with 100 TCID50 H1N1 virus. After adsorbing for 2h, the cells were divided into H1N1 infected group（group B）, oseltamivir group（group C, 0.75μg/m L）, high dose of baicalin group（group D, 3.96μg/m L）, low dose of baicalin group（group E, 0.99μg/m L）, and with normal A549 cells as the control group（group A）. The differential expression of inflammatory factors in cells of each groups were determined by using gene chip technology, and the m RNA and protein experience of the target factors in cells was tested by using RT-PCR and Western blotting methods. Results： Compared with group A, the m RNA expression of IL-1β, IL-6, IL-8, TNF-α and RANTES of cells in group B was obviously up-regulated（P0.01）, while the m RNA expression of the above cytokines was down-regulated, and the m RNA expression of IL-10 was obviously up-regulated after treating（P0.05, P0.01）. The test results of RT-PCR and Western blotting were consistent with the gene chip result. Conclusion： The overexpression of inflammatory factors induced by infection of influenza virus would bring damage to cells, and the above changes could be improved by baicalin which has obvious anti-infection effect.

关 键 词：黄芩苷 流感病毒H1N1 人肺腺癌A549细胞 炎性反应 细胞因子 基因芯片 

分 类 号：R285[医药卫生—中药学]