江西荆芥不同部位及荆芥炭中熊果酸及齐墩果酸含量测定  

Contents determination of ursolic acid and oleanolic acid in different parts of Jiangxi Herba Schizonepetae and carbonized Herba Schizonepetae

作　　者：蒋以号[1] 蒋待泉 武孔娟 刘丽芳[1] 彭林丽 

机构地区：[1]南昌大学资源环境与化工学院,南昌330029

出　　处：《中华中医药杂志》2016年第3期1068-1070,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江西省科技支撑计划资助项目(No.20112BBG70019);南昌市科技计划资助项目(No.2011-ZCSF-CXYWJZYXDH-006)~~

摘　　要：目的:用HPLC法测定江西荆芥不同部位及荆芥炭中熊果酸与齐墩果酸的含量。方法:色谱柱:Hypersil ODS2-C18柱(250mm×4.6mm,5μm);流动相:甲醇∶0.1%磷酸水溶液=85∶15(v/v);柱温:30℃;检测波长:210nm;流速:0.8m L/min;进样量:10μL。结果:齐墩果酸在0.104-0.624μg范围内线性关系良好(r=0.9988);熊果酸在0.416-2.496μg范围内线性关系良好(r=0.9997)。熊果酸与齐墩果酸含量由高至低依次均为:荆芥穗>荆芥茎>荆芥全草>荆芥炭>荆芥根。结论:江西荆芥中齐墩果酸和熊果酸含量较高,且不同部位中的含量相差较大;该方法分离效果良好,简便易行,重现性好,可作为此类成分检测的有效方法。Objective： To determine the contents of ursolic acid and oleanolic acid in the different parts of Jiangxi Herba Schizonepetae and carbonized Herba Schizonepetae by HPLC. Methods： The conditions of HPLC for contents determination of ursolic acid and oleanolic acid in different parts of Jiangxi Herba Schizonepetae and carbonized Herba Schizonepetae were as follows： hypersil ODS2-C18 chromatographic column（4.6mm×250mm, 5μm） was used; the mobile phase comprised 0.1% methanol and 0.1% phosphoric acid（v/v=85/15）; the analysis was performed at a flow rate of 0.8m L/min with column temperature was 30℃; the injection volume was 10 μL, and the determination wavelength was 210 nm. Results： The calibration curves were linear in the range of 0.104-0.624μg for oleanolic acid（r=0.9988） and 0.416-2.496μg for ursolic acid（r=0.9997）. The contents of ursolic acid and oleanolic acid from high to low were, in order, schizonepetaspike, schizonepeta stem, herba of schizonepetae, charred schizonepeta, schizonepeta root. Conclusion： The contents of oleanolic acid and ursolic acid were higher, and there were large differences in contents in the different parts of Jiangxi Herba Schizonepetae and carbonized Herba Schizonepetae. The method might be an effective method for determination of this kind of composition with advantages as simple, good effects and fine reproducibility.

关 键 词：荆芥 齐墩果酸 熊果酸 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]