肝郁型CNP大鼠模型的建立及免疫学的初步研究  

Preliminary study on immunology and establishment of CNP rat model with stagnation of liver qi

作　　者：孙洪福[1] 刘燕[2] 颜培正 王树增[3] 张庆祥[2] 

机构地区：[1]山东中医药大学附属医院泌尿外科,济南250014 [2]山东中医药大学基础医学院,济南250355 [3]山东省立医院南院针灸科,济南250022

出　　处：《中华中医药杂志》2016年第3期1074-1076,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2013051);央财基础特色项目(No.220207)~~

摘　　要：目的:通过理论、临床及免疫学探讨,建立肝郁型慢性非细菌性前列腺炎(CNP)大鼠模型。方法:采用Wistar雄性大鼠50只,分为:CNP模型组37只、前列腺蛋白制备组5只、空白组8只。先采用弗氏免疫佐剂法制备CNP模型,观察肿瘤坏死因子-α(TNF-α)、白介素-8(IL-8)变化;然后对CNP模型组采用束缚法制备肝郁型CNP模型,观察TNF-α、IL-8变化。结果:正常饲养的CNP模型组TNF-α、IL-8浓度较空白组明显升高(P<0.05),肝郁型CNP模型组TNF-α、IL-8浓度明显高于正常饲养的CNP模型组(P<0.05)。结论:弗氏免疫佐剂法结合束缚法能够制备肝郁型CNP模型,肝郁能够加重CNP炎性改变。Objectives： To establish CNP rat model with stagnation of liver-qi through discussion of theory, clinic and immunology. Methods： Fifty male Wistar rats were divided into CNP model group（37 rats）, prostate-protein preparation group（5 rats）, and control group（8 rats）. The CNP models were established by using Freund＇s adjuvant method, and the changes in the levels of TNF-α and IL-8 were observed. On this basis, combined with the constraint method, the CNP models with stagnation of liver-qi were established, and the changes in the levels of TNF-α and IL-8 were observed again. Results： the concentration of TNF-α and IL-8 of rats in CNP model group increased significantly, while the concentrations of TNF-α and IL-8 levels of rats in CNP with stagnation of liver-qi group were obviously higher than those of CNP model group. Conclusion： Freund＇s adjuvant method combined with constraint method could be used to establish CNP rat model with stagnation of liver-qi, and the stagnation of liver-qi could aggravate the inflammatory changing of chronic nonbacterial prostatitis.

关 键 词：临床 免疫学 肝郁 慢性非细菌性前列腺炎 病证结合模型 

分 类 号：R277.5[医药卫生—中医学] R-332