闽南地区HLA-B27基因亚型与强直性脊柱炎相关性研究  

The association of HLA-B27 genotype and subtype with ankylosing spondylitis in Southern Fujian

作　　者：范春梅[1] 黄荣富[1] 陈根旺 

机构地区：[1]福建医科大学附属第二医院东海院区检验科,福建泉州362000

出　　处：《中国微生态学杂志》2016年第3期256-258,共3页Chinese Journal of Microecology

基　　金：泉州市科技项目(Z【2014】0008)

摘　　要：目的对闽南地区人群中HLA-B27阳性标本进行回顾性调研及分型检测,探讨闽南地区HLAB27亚型分布情况,分析HLA-B27亚型与强直性脊柱炎(AS)的相关性。方法采用PCR-SBT(测序法)基因分型技术,对流式细胞术(FCM)检测为阳性的186例标本进行HLA-B*27基因分型。结果 186例HLA-B27阳性(FCM法)标本中,再用PCR-SBT法检测,HLA-B*27基因型均为阳性;而基因亚型分布,174例为B*27:04亚型,占93.5%,有8例B*27:05亚型,占4.3%,B*27:15亚型2例,占1.1%,另外B*27:06和B*27:07各1例,均占0.5%。186例中有103例确诊为AS患者,其中98例为B*27:04亚型,占95.1%,3例为B*27:05亚型,占2.9%,B*27:07和B*27:15亚型各1例,均占1.0%。结论闽南地区HLA-B27阳性的强直病患者以B*27:04为主,其次为B*27:05。AS患者与其他B27基因携带者,在亚型分布上没有明显的差异。Objective To investigate the relationship between HLA-B27 genotypes and ankylosing spondylitis（AS）by detecting the distribution of HLA-B27 genotype in the population of Southern Fujian.Methods PCRSBT genotyping method was used to analyze the genotypes of 186 HLA-B27 samples which were collected from the Second Affiliated Hospital of Fujian Medical University and detected as HLA-B＊27positive by FCM.Results All the 186 samples were detected as HLA-B＊27positive by PCR-SBT,among which 174 cases were B＊27：04 while 8 were B＊27：05subtype.103 cases were diagnosed as AS,among which B＊27：04were predominant.Conclusion HLA-B＊27：04is the predominant subtype in the AS patients in Southern Fujian.There are no significant differences in the distribution of B27 subtypes between AS patients and non-AS patients.

关 键 词：强直性脊柱炎 HLA-B27 PCR-SBT HLA-B＊27:04 HLA-B＊27:05 

分 类 号：R593.23[医药卫生—内科学]