貉源伪狂犬病毒Rac 1株的分离鉴定  

Isolation and identification of nyctereutes pseudorabies virus Rac 1 strain

作　　者：刘曜综 芮萍[1] 马增军[1] 马芮[2] 杨卫军 张珍珍 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛066004 [2]吉林大学临床医学院,吉林长春130021 [3]秦皇岛市农产品质量安全监督检验中心,河北秦皇岛066000

出　　处：《中国预防兽医学报》2016年第3期202-205,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：河北省科技支撑项目(13226609D);河北省畜牧局计划项目(2011-1-19)

摘　　要：为分析近年我国人工养殖貉出现疑似伪狂犬病症状的病因,本研究从病死的貉脑组织中分离到一株病毒并对其进行了系统鉴定。结果显示,该病毒分离株在MDCK细胞中传代呈现出疱疹病毒特征的细胞病变。电镜观察可见直径150 nm^180 nm,带有囊膜的病毒粒子。以猪抗伪狂犬病病毒(PRV)阳性血清为一抗,对病毒分离株进行间接免疫荧光试验,结果呈阳性。该病毒分离株对氯仿、胰酶敏感,在pH5~9的环境中稳定,对热具有较强的耐受性。动物试验均出现典型的伪狂犬病症状。分子生物学检测分析结果表明,本研究所分离的病毒株为PRV,并将其命名为貉源PRV Rac 1株。本研究为预防和控制貉伪狂犬病提供了依据。A disease suspected with pseudorabies had occurred in a large number of raccoon dog herds in China since 2014. To investigate the causative agent, we isolated a virus fi＇om the brain tissue of a dead raccoon dog and the virus produced the herpes virus like typical cytopathogenic effect in Madin-Darby canine kidney （MDCK） cells. The mature virions with envelope in a diameter of 150 nm to 180 nm were observed under transmission electron microscopy. The virus was also sensitive to chloroform and trypsin, and tolerance to pH 5 to 9. In addition, the positive results for pseudorabies virus （PRV） were detected by immunofluorescence assay and PCR. Furthermore, the rabbits and mice injected with this virus developed typical clinical symptoms of pseudorabies when artificially infected with the virus. All the evidences indicated that the isolated virus was PRV and named Rac 1. This study provided references for raccoon dog pseudorabies prevention and control.

关 键 词：貉 伪狂犬病毒 分离鉴定 GE基因 GC基因 

分 类 号：S852.65[农业科学—基础兽医学]