乌拉尔图小麦NAC转录因子的筛选与分析  

Identification and analysis of the NAC transcription factor family in Triticum urartu

作　　者：马建辉[1] 仝豆豆 张文利[1] 张黛静[1] 邵云[1] 杨云[1] 姜丽娜[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《遗传》2016年第3期243-253,共11页Hereditas（Beijing)

基　　金："十二五"国家科技支撑计划项目(编号:2013BAD07B14;2012BAD14B08)资助~~

摘　　要：NAC是植物特有的具有多种功能的一类转录因子,广泛参与植物的生长发育、器官建成及抗逆境胁迫等反应。目前有关NAC转录因子的研究主要针对模式植物(如拟南芥和水稻),而在小麦中的研究相对较少。本文利用生物信息学方法,获得乌拉尔图小麦(Triticum urartu)NAC转录因子家族基因的全长序列,并对其进化关系、生物学功能、染色体定位以及基因复制等进行预测与分析,同时利用荧光定量PCR验证相关转录因子在非生物胁迫下的表达模式。结果显示,共筛选得到87个乌拉尔图小麦全长NAC转录因子,通过进化树分析将其分为7个亚族,其中39个NAC转录因子被定位在7条染色体上。通过基因复制分析发现,有5对NAC转录因子基因发生了复制。进一步通过荧光定量验证4个NAC转录因子在非生物胁迫下的表达模式,发现4个转录因子均受不同胁迫而上调表达。NAC transcription factors are one of plant-specific gene families with diverse functions, and they regulate plant development, organ formation and stress responses. Currently, the researches about NAC transcription factors mainly focus on model plants, Arabidopsis and rice, whereas such studies are hardly reported in wheat and other plants. In this study, the full-length coding sequences（CDS） of NAC transcription factors from Triticum urartu（TuNAC） were identified through bioinformatic analysis. Their biological function, evolutionary relationship, gene duplication and chromosomal locations were further predicted and analyzed. The quantitative real-time PCR（q RT-PCR） assay was used to verify the expression pattern of abiotic-related Tu NAC transcription factors. A total of 87 Tu NAC transcription factors with full-length CDS were identified, which were divided into seven subgroups through phylogenetic analysis. Thirty-nine Tu NAC transcription factors were located on seven chromosomes, and five pairs of Tu NAC transcription factors were duplicated. The expression of four Tu NAC transcription factors was consistently increased under diverse abiotic stress by q RT-PCR assay. Our study thus provides basis for further functional investigations of Tu NAC transcription factors.

关 键 词：乌拉尔图小麦 NAC转录因子 进化分析 非生物胁迫 

分 类 号：S512.1[农业科学—作物学]