甘蔗与斑茅割手密复合体(GXAS07-6-1)杂交后代的染色体遗传分析  

Genetic Analysis of Chromosomes for the Progeny Between Sugarcane(Saccharum spp.)and Intergeneric Hybrid Complex(Erianthus arundinaceus×Saccharum spontaneum)

作　　者：黄玉新[1,2] 罗霆[2,3] 刘昔辉[2,3] 周珊[2,3] 杨翠芳[2,3] 高轶静[2,3] 杨翠凤[1,2] 段维兴[2,3] 周会[2,3] 张革民[2,3] 李杨瑞[2,3] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西甘蔗遗传改良重点实验室,广西南宁530007 [3]广西农业科学院甘蔗研究所农业部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007

出　　处：《热带作物学报》2016年第2期220-225,共6页Chinese Journal of Tropical Crops

基　　金：国家现代农业产业技术体系(No.CARS-20-1-3);广西自然科学基金项目(No.2014GXNSFBA118115);广西农业科学院基本科研业务专项项目(桂农科2013YT06;2014YZ03;2015YT04;2014YQ02;2015JZ96);广西"八桂学者"专项经费

摘　　要：本研究的目的是明确甘蔗与斑割（斑茅割手密）复合体杂交后代F_1材料的染色体核型构成和遗传行为,为有效利用甘蔗野生种质提供细胞遗传学的理论依据。采用甘蔗根尖细胞酶解去壁低渗法分别对3个不同甘蔗品种（系）与斑割复合体GXAS07-6-1的杂交后代进行染色体数目鉴定和核型分析。结果表明：各亲本和后代材料染色体数目在不同细胞中不恒定,染色体变幅为6~11条。粤糖93-159与GXAS07-6-1的F1材料GXASF108-1-1染色体核型公式为2n=94=91 m＋3 sm;桂糖01-53与GXAS07-6-1的F_1材料GXASF108-2-28染色体核型公式为2n=98=88 m＋10 sm（1SAT）;桂糖02-761与GXAS07-6-1的F_1材料GXASF108-3-7染色体核型公式为2n=92=87m＋5 sm;3个F1材料的核型均为2B型。推断甘蔗与斑割复合体杂交亲子间的染色体基本按＂n＋n＂方式传递,同时可能存在部分染色体加倍现象,它们的杂种F_1核型均为较原始的染色体2B类型。To incorporat e genes from wild germplasm for laying a good cytogenetical foundation of sugarcane,karyotype composition and genetic behavior of chromosome were explored for the F_1 progeny between sugarcane（ Saccharum spp.）and intergeneric hybrid（Erianthus arundinaceus ×Saccharum spontaneum）. Chromosome number calculation and karyotype analysis were carried out for the progeny between 3 clones（Saccharum spp.）and GXAS07-6-1（Erianthus arundinaceus ×Saccharum spontaneum）by the means of cell wall degradation hypotonic smear method. The somatic chromosome numbers of all accessions were non-constant and the amplitude was 6-11.The somatic chromosome karyotypic types of F_1 progeny for 3 crosses were 2n=94=91 m＋3 sm, 2n=98=88 m＋10 sm（1SAT）, and 2n=92=87 m＋5 sm, respectively. The ＂n＋n＂ transmission and 2B karyotype of F_1 progeny from sugarcane and intergeneric hybrid were indicated. The chromosomes of sugarcane and intergeneric hybrid complex（Erianthus arundinaceus ×Saccharum spontaneum）were transmitted in the mode ＂ n ＋n＂ but doubling possibly existed. The karyotypes of these three F_1 progenies belong to the primitive 2B type.

关 键 词：甘蔗 斑茅割手密复合体 核型分析 染色体传递 

分 类 号：S566.1[农业科学—作物学]