基于线粒体COII-LrRNA基因对肾形肾状线虫种内群体的遗传多样性分析  

Genetic Diversity of Intra- species Populations of Rotylenchulus reniformis Based on Mitochondrial COII-Lr RNA Gene

作　　者：邓艳凤[1,2] 田忠玲 郑经武[1] 

机构地区：[1]浙江大学农业与生物技术学院生物技术研究所,杭州310058 [2]苏州出入境检验检疫局,苏州215021

出　　处：《农业生物技术学报》2016年第4期593-599,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31371921);国家重点基础研究发展计划(973计划)(No.2013CB127501)

摘　　要：肾形肾状线虫（Rotylenchulus reniformis）是一种植物半内寄生线虫,分布于世界的热带和亚热带地区,是许多蔬菜和热带果树重要的病原线虫。为明确该线虫种内群体的遗传变异,本研究采用序列分析法,对采自浙江（ZJ）、福建（FJ）和重庆（CQ）3个地区的肾形肾状线虫的线粒体COII-Lr RNA基因序列进行比较。结果表明,肾形肾状线虫线粒体COII-Lr RNA基因片段序列为557~563 bp,AT含量为85.5%,明显高于GC含量。序列分析所得3个群体总的变异位点数、单倍型数、单倍型多样性指数（haplotype diversity,Hd）和核苷酸多样性指数（nucleotide diversity,π）分别为176、40、0.946和0.157 4。分子方差分析（analysis of molecular variance,AMOVA）结果显示,这3个肾形肾状线虫群体间遗传分化系数为0.058 15,遗传分化程度中等,没有明显的地理隔离。遗传变异结果显示,94.18%的变异来自群体内个体间,只有5.82%的变异发生在群体间。结果说明,我国肾形肾状线虫种群内COII-Lr RNA基因序列变异较明显,具有丰富的遗传多样性,且对环境变化的适应能力较强。研究结果丰富了我国肾形肾状线虫的系统发育信息,为肾形肾状线虫危害植物的内在遗传因素的研究提供了资料。Rotylenchulus reniformis is a semi-endoparasitic nematode, which widely distributes in tropical and subtropical area of the world, and is pathogenic nematodes of many vegetables and tropical fruit trees. Our research was focus on identification and phylogenetic relationship among different geographical populations of reniform nematode. To characterize the genetic diversity of intra-species populations of Rotylenchulus reniformis, sequence variations of COII- LrRNA mitochondrial DNA （mtDNA） between individuals and 3 intra- species populations of reniform nematode from Zhejiang （Z J）, Fujian （F J） and Chongqing （CQ） were analyzed based on DNA sequence analysis technology. The result showed that the length of COII-LrRNA gene region ranged from 557 to 563 bp using primer 1108 and C2F3-R. The average content of A, T, C and G were 32.6%, 52.9%, 4.5% and 10.0%, respectively. The content of A＋T was 85.5%, and it was much higher than that of G＋ C content. Obvious genetic diversity was found from the amplified region. All 389 conserved sites, 176 variation sites, 51 parsimony-informative sites and 125 singleton sites were identified. There were 40 haplotypes among the 64 sequences, which suggested a high level of heteroplasmy in R. reniformis, and 17 haplotypes were found in ZJ population, 13 haplotypes in CQ population and 12 haplotypes in FJ population. The number haplotype diversity （Hd）, and nucleotide diversity （π） of 3 population were 0.946 and 0.157 4, respectively. The analysis of molecular variance （AMOVA） showed that the Fst （F-statistics） of coefficient of genetic differentiation was 0.058 15 （P〈0.01）, which placed these isolates into a moderate range of genetic differentiation and no obvious geographic isolation. The variation within populations was 94.18% and only 5.82% of the variation occurred among the 3 intra-species populations. In general, the variation among COI1- LrRNA gene sequence of reniform nematodes in China was obvious, which indicated that reniform nema

关 键 词：肾形肾状线虫 线粒体DNA(mtDNA) COII-LrRNA基因 遗传多样性 

分 类 号：Q933[生物学—微生物学]