小鼠急性铜绿假单胞菌肺部感染中白细胞介素17的变化及意义  

Changes and Effects of IL-17 in Mice with Acute Pseudomonas Aeruginosa Lung Infection

作　　者：陈真真[1] 张波[2] 唐忠志[1] 徐西琳[1] 

机构地区：[1]广州军区武汉总医院急诊科,湖北武汉430070 [2]广州军区武汉总医院呼吸内科,湖北武汉430070

出　　处：《华南国防医学杂志》2016年第1期5-8,18,共5页Military Medical Journal of South China

摘　　要：目的以急性铜绿假单胞菌肺部感染的小鼠为研究对象,探讨白细胞介素17(interleukin-17,IL-17)在铜绿假单胞菌肺部感染中的变化及可能机制。方法经气道内注入铜绿假单胞菌建立急性肺部感染小鼠模型,随后收集3d的动物肺组织、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及外周血,采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RQ-PCR)和酶联免疫吸附剂测定(enzyme-linked immunosorbent assay,ELISA)方法检测IL-17以及炎性因子IL-1β、粒细胞集落刺激因子(granulocyte colony stimulating factor,G-CSF)的表达水平。结果急性铜绿假单胞菌肺部感染后的小鼠肺组织IL-17的mRNA水平显著高于对照组,肺泡灌洗液中IL-17的蛋白水平也显著高于对照组,两组比较差异具有统计学意义(P<0.05)。感染组肺泡灌洗液中IL-1β及G-CSF的表达水平在3 d内显著高于对照组,两组比较差异具有统计学意义(P<0.05)。结论 IL-17可能通过其下游因子IL-1β和G-CSF的作用使气道中性粒细胞增加,从而在早期发挥对急性铜绿假单胞菌肺部感染的保护作用。Objective To explore the changes and effects of interleukin 17（IL-17）in mice with acute pseudomonas aeruginosa lung infection.Methods The mice mode with acute lung infection was established by slow injection of pseudomonas aeruginosa into the mice trachea.The expression of IL-17,IL-1β and granulocyte colony stimulating factor（G-CSF）were detected in the lung tissues and bronchoalveolar lavage fluid（BALF）by enzyme-linked immunosorbent assay（ELISA）detection and real-time quantitative polymerase chain reaction（RQ-PCR）within 3 days.Results The IL-17 protein in BALF and IL-17 mRNA expression were observed in infection group significantly higher than that of control group after pseudomonas aeruginosa infection（P〈0.05）.The BALF level of IL-1β and G-CSF was significantly higher than that of control group within 3 days（P〈0.05）.Conclusion IL-17 may increase the number of neutrophils by IL-1β and G-CSF to protect acute pseudomonas aeruginosa lung infection in the early.

关 键 词：白细胞介素17 铜绿假单胞菌 肺部感染 免疫应答 

分 类 号：R563[医药卫生—呼吸系统]