TLC-HPLC检测三黄连合剂的有效成分  

Determination of effective components in Chinese herbal compound Sanhuanglian by TLC-HPLC

作　　者：杨剑[1] 牛晓艺 黄明[1] 梁万文 胡庭俊[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,南宁530021

出　　处：《南方农业学报》2016年第2期312-318,共7页Journal of Southern Agriculture

基　　金：国家现代农业产业技术体系广西罗非鱼创新团队建设项目(GXYB-02);广西水产畜牧兽医局科研计划项目(桂渔牧科201452021)

摘　　要：【目的】以薄层色谱法—高效液相色谱法（TLC-HPLC）检测三黄连合剂的有效成分,为制定其质量标准及后期临床推广应用提供理论依据。【方法】采用TLC对三黄连合剂中的主要组分黄连、黄芩和连翘进行定性鉴别,并以HPLC对其主要有效成分黄芩苷、盐酸小檗碱和连翘苷进行含量测定。【结果】三黄连合剂TLC色谱中的图像斑点清晰,与标准品对照斑点对齐,颜色一致。HPLC测定三黄连合剂中黄芩苷、连翘苷和盐酸小檗碱分别在75.00-1200.00μg/m L（r=0.9997）、4.17-66.67μg/m L（r=0.9998）、9.38-150.00μg/m L（r=0.9999）范围内线性关系良好,平均回收率分别为113.92%、100.18%和100.40%,其含量分别为5.03、0.25和0.27 mg/m L。【结论】TLC-HPLC可作为三黄连合剂的质量控制方法,且操作简便、重现性良好。【Objective】In order to theoretical support for formulating quality standard of Chinese herbal compound Sanhuanglian and its late-stage clinical application, the effective components in Chinese herbal compound Sanhuanglian were detected by thin layer chromatography-high performance liquid chromatography（TLC-HPLC）. 【Methods】The main components of Chinese herbal compound Sanhuanglian, Coptis chinensis, Scutellaria baicalensis and Forsythia suspensa,were identified qualitatively by TLC. And the contents of baicalin, berberine hydrochloride and phillyrin was determined by HPLC. 【Result】The TLC results showed that, the spots of tested samples were clear and tidy, and uniform with those of standards. The HPLC results showed, there were good linear relations for baicalin, berberine hydrochloride and phillyrin at ranges of 75.00-1200.00 μg/m L（r =0.9997）, 4.17-66.67 μg/m L（r =0.9998）, 9.38-150.00 μg/m L（r =0.9999）,respectively, the average recovery rates were 113.92%, 100.18% and 100.40%, respectively, and the contents were5.03,0.25 and 0.27 mg/m L, respectively. 【Conclusion】TLC-HPLC is simple, accurate and repeatable, which can be used to evaluate and control quality of Chinese herbal compound Sanhuanglian.

关 键 词：三黄连合剂 有效成分 质量标准 薄层色谱法(TLC) 高效液相色谱法(HPLC) 

分 类 号：S853.73[农业科学—临床兽医学]