不同光源对抑郁模型大鼠行为学及褪黑素影响探讨  

Effects of different light on the ethology and melatonin secretion in depressive rats

作　　者：周书喆 党卫民[1] 张国义[2] 周天航[1,3] 林健[4,5] 司天梅[1] 李继涛[1] 何仲恺[2] 钟灿涛[2] 王生[6] 赵丽[3,7] 王永志[2] 魏伟[2] 黄振烈[4] 张阔[8] 陈志忠[2] 刘薏[1] 刘洋[7] 赵荣生[7] 孙海明[8] 李思恒[3] 牛荣峰 童玉珍[2] 马燕桃[1] 于欣[1] 

机构地区：[1]北京大学第六医院,北京大学精神卫生研究所,卫生部精神卫生学重点实验室(北京大学),国家精神心理疾病临床医学研究中心(北京大学第六医院),北京100191 [2]北京大学物理学院,北京大学宽禁带半导体研究中心,100871 [3]北京大学基础医学院,100191 [4]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [5]广东省深圳市龙华新区疾病预防控制中心,深圳518109 [6]北京大学公共卫生学院,100191 [7]北京大学第三医院,100191 [8]北京大学实验动物科学部,100871 [9]北京大学生命科学学院,100191

出　　处：《中国职业医学》2016年第1期8-14,共7页China Occupational Medicine

基　　金：国家高技术研究发展计划(863计划,2011AA03A113)

摘　　要：目的观察节能灯、白炽灯和生物节律光源对抑郁模型大鼠行为学的影响,初步探讨其对褪黑素的影响机制。方法无特定病原体级健康雌性6周龄SD大鼠40只,适应性饲养后,随机取30只于11~14周龄时采用双侧卵巢切除联合孤养、慢性不可预知温和应激的复合式方法建立抑郁模型后,随机分为节能灯、白炽灯和生物节律灯干预组,每组10只,于17周龄时分别予相应的光源光照干预,为期3周;另10只大鼠为对照组,常规饲养。在17、19、20和21周龄时称量大鼠体质量,在7、14、20周龄时分别采用糖水偏好实验和旷场实验对大鼠进行行为学测试,在21周龄时测定大鼠1个暗周期（19：30）至明周期（08：30）7个时间点的周围血褪黑素水平,每组每个时间点随机测定1只大鼠。结果光照干预前的17周龄时,4组大鼠体质量差异无统计学意义（P〉0.05）;光照干预后,3个干预组大鼠体质量在17~20周龄均呈随时间增加而增长趋势（P〈0.05）;21周龄时4组大鼠体质量分别与同组20周龄比较,差异均无统计学意义（P〉0.05）。7周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义（P〉0.05）;造模后未予光照干预前的14周龄时,3个干预组大鼠糖水消耗量和站立评分均低于对照组（P〈0.05）,3个干预组大鼠上述2个指标差异均无统计学意义（P〉0.05）;光照干预结束后20周龄时,4组大鼠糖水消耗量和站立评分差异均无统计学意义（P〉0.05）。3个干预组大鼠周围血褪黑素高峰值均高于对照组;节能灯和节律灯干预组大鼠周围血褪黑素高峰分别较对照组提前或延后2 h出现,而白炽灯干预组与对照组大鼠周围血褪黑素高峰出现时间相近。结论生物节律灯与节能灯、白炽灯对抑郁模型大鼠行为均有改善作用,3种光照干预效果相近;生物节律灯干预可延迟周围血褪黑素高峰出现时间。Objective To observe the impact of energy saving light,incandescent light and circadian light on the ethology of depressive rats and explore its possible mechanism on affecting the secretion of melatonin. Methods Thirty rats aged 6weeks were randomly selected from 40 specific pathogen free health female SD rats after they adapted to the living environment,depressive rat models were established in the rats by bilateral ovariectomy combined with isolated living and chronic unpredictable mild stress stimulation at the age of 11-14 weeks. Then these 30 ovariectomized rats were randomly divided into 3 intervention groups,including an energy saving light group,an incandescent light group and a circadian light group,with 10 rats in each group. The rats in these 3 groups were given specific experimental light intervention for 3 weeks respectively at the age of 17 weeks. The other 10 rats were raised in conventional environment as the control group. Their body weights were measured at the age of 17,19,20 and 21 weeks. The ethology tests were carried out by sucrose preference test and the open-field test at the age of 7,14 and 20 weeks respectively. The melatonin levels in peripheral blood of 7 time points from 19： 30 to 8： 30 were measured in the rats at age of 21 weeks. One rat in each group at every time point was randomly selected for examination. Results At the age of 17 weeks before light-intervention,the body weights of rats in 4 groups showed no significant difference（ P〈0. 05）. After light-intervention,at the age of 17-20 weeks,the body weights of rats in 3 intervention groups were gradually increased with the increase of age（ P〈0. 05）.There was no significant difference between body weights of rats at the age of 21 weeks and those at the age of 20 weeks in each group（ P〈0. 05）. At age of 7 weeks,no significant differences were found in sucrose consumption and standing scores among these 4 groups（ P〈0. 05）. After the depressive models were established,at the age of 14 weeks before light

关 键 词：生物节律光源 抑郁模型 抑郁症 行为学 褪黑素 糖水偏好实验 旷场实验 大鼠 

分 类 号：R454.2[医药卫生—治疗学] R135.99[医药卫生—临床医学]