火麻仁薄层色谱鉴别用对照提取物的研制与应用  

Study on the Preparation and Application of Cannabis Fructus TLC Reference Extract

作　　者：邓仕任[1] 蔡明宸[1] 夏林波[1] 王鑫[1] 朱夏敏 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《广州化工》2016年第5期71-73,共3页GuangZhou Chemical Industry

基　　金：2015年版中国药典一部科研立项(火麻仁药材及饮片);辽宁省高等学校优秀人才支持计划(LJQ2015070);全国中药饮片炮制规范(第二批)研究任务(2015-YP-24)

摘　　要：研制被薄层色谱鉴别用火麻仁对照提取物,以替代对照药材用于火麻仁的薄层鉴别。从制备工艺考察、提取物稳定性影响因素考察以及提取物性状等方面制订薄层色谱鉴别用火麻仁对照提取物的制备和保存方法,并比较对照药材与对照提取物在薄层鉴别中的应用结果。结果表明：制备方法为：火麻仁药材粉末（过二号筛）加25倍乙醚脱脂后,药渣加10倍甲醇加热回流提取1 h,滤过,减压蒸去甲醇,所得浸膏用10倍甲醇复溶,加入与浸膏等重量的柱层析硅胶（200~300目）,混匀,减压蒸干甲醇,过九号筛即得。该对照提取物与火麻仁药材的薄层鉴别效果一致。本研究制备的火麻仁薄层鉴别用对照提取物稳定可靠,可以替代火麻仁对照药材用于火麻仁及其成方制剂的质量控制分析。Cannabis fructus TLC reference extract was prepared and its application in the quality control of Cannabis fructus and preparations as a reference drug was explored. Cannabis fructus TLC reference extract was prepared by opting processing route and investigating stability influencing factors. The application to TLC identification items of Cannabis fructus was performed according to the Chinese Pharmacopoeia 2010 edition. Results showed that Cannabis fructus TLC reference extract can play the same role as Cannabis fructus reference drug with the same quantity and quality. Cannabis fructus TLC reference extract for TLC identification can be used for quality control analysis of Cannabis fructus and preparations. The research provided a foundation for further development of TCM reference extracts.

关 键 词：火麻仁 薄层色谱鉴别 对照提取物 质量控制 

分 类 号：R284.1[医药卫生—中药学]