四氧化三铁磁性纳米颗粒人工模拟酶在快速检测二硝基甲苯中的研究及应用  

Fe_3O_4 Magnetic Nanoparticles as Peroxidase Mimetics Used in Colorimetric Determination of 2,4-Dinitrotoluene

作　　者：聂冬霞[1] 施国跃[2] 于妍妍[3] 

机构地区：[1]上海市农业科学院,农产品质量标准与检测技术研究所,上海201403 [2]华东师范大学化学系,上海200241 [3]徐州医学院药学院,徐州221004

出　　处：《分析化学》2016年第2期179-185,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.31301494)项目资助~~

摘　　要：通过共沉淀法制备了具有类过氧化物酶催化活性的四氧化三铁磁性纳米颗粒(Fe_3O_4 MNPs)。研究表明,Fe_3O_4 MNP_s可以催化H_2O_2氧化2,2'-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)(ABTS)二铵盐,生成有色的氧化态ABTS,同时也可催化H_2O_2氧化二硝基甲苯(DNT)的反应,消耗反应物H_2O_2。基于上述原理,构建了Fe_3O_4 MNP_s-ABTS-H_2O_2-DNT反应体系,用于检测DNT。结果表明,氧化态ABTS在417nm处的吸收值随着DNT含量的增加而降低,与DNT浓度在5×10^(-7)~2.0×10^(-5)mol/L范围内呈良好的线性关系,检测限为1.5×10^(-7)mol/L(S/N=3)。采用紫外-可见分光光度法对Fe_3O_4 MNP_s-ABTS-H_2O_2-DNT体系的反应机理、反应条件等进行了深入探讨。所建立的硝基苯类化合物含量的比色分析方法简便、快速、灵敏、可靠,对环境水样中硝基苯类化合物的在线监测具有潜在的应用价值。Fe3O4 magnetic nanoparticles （Fe3O4 MNPs） were prepared by co-precipitation method, which possessed intrinsic peroxidase-like activity. It was found that the as-prepared Fe3O4 MNPs could not only catalyze the oxidation of peroxidase substrate, diammonium 2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulfonate） （ABTS） by H202 to produce the colored product with maximum absorbance at 417 nm, but also catalyze the oxidation of 2,4-dinitrotoluene （DNT） by H202 and thus consume H2O2 in the reaction system. On the basis of the reaction described above, a novel colorimetric reaction system based on Fe3O4 MNPs-ABTS-H2O2-DNT was established for determination of trace amount of DNT. Some key influencing factors of the proposed Fe3O4 MNPs-ABTS-H202-DNT system were systematically discussed and optimized. Under the optimal conditions, the absorbanee of the system at 417 nm displayed a good linear response with DNT concentration in the range from 5× 10-7 to 2.0 × 10-5 mol/L with a detection limit of 1.5× 10-7 mol/L （ S/N= 3 ）. These results demonstrated that the constructed colorimetric method was simple, rapid and sensitive enough for colorimetric detection of DNT, which would have a great potential in the application of on-line monitoring of DNT in environmental water samples.

关 键 词：二硝基甲苯 四氧化三铁磁性纳米颗粒 过氧化物模拟酶 分光光度法 

分 类 号：O657.3[理学—分析化学] TB383.1[理学—化学]