体外仿生法研究牡蛎和蛤仔体内微量金属元素的生物可给性  

Bioaccessibility of Microamount Metal Elements in Oyster and Cam Samples Using in vitro Digestion

作　　者：王增焕[1] 王许诺[1] 林钦[1] 陈成桐[1] 陈瑛娜[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东省渔业生态环境重点实验室,广州510300

出　　处：《分析化学》2016年第2期247-251,共5页Chinese Journal of Analytical Chemistry

基　　金：国家科技支撑计划课题(No.2009BADB7B02);农业部水产品加工重点实验室开放基金(No.NYJG201306);中央级科研院所基本科研业务费专项资金(Nos.2013YD08,2015TS16)资助~~

摘　　要：采用体外全仿生消解方法,模拟人体的消化道环境,对牡蛎和蛤仔样品进行仿生消解,分析牡蛎和蛤仔体内微量元素的生物可给性。以原子吸收光谱法测定胃、肠仿生提取液中微量元素的含量。结果表明,牡蛎胃提取液中Fe,Cu,Zn,Cd的含量明显高于肠提取液中的含量,蛤仔胃提取液中Fe,Cu的含量与肠提取液中的含量没有明显差异,而Zn,Cd的含量远高于其在肠提取液中的含量。经全仿生消解后,牡蛎体Fe,Cu,Zn,Cd的可给性分别为60.2%,83.6%,83.1%,76.8%;蛤仔Fe,Cu,Zn,Cd的可给性分别为46.3%,86.3%,85.3%,87.7%。本研究结果为进一步研究牡蛎和蛤仔对金属元素的累积和迁移、生物可利用性,Cd等有害元素对食用者的健康风险评估,提供技术支持和理论依据。A bionic method, in vitro digestion, was used for the pretreatment of oyster and clam samples. The oyster and clam samples were digested at 37℃ under the action of bionic digestive juice including saliva, gastric juice and intestinal juice. The contents of trace metals in in vitro digestion extracts （gastric juice extracts and intestinal juice extracts） were determined by atomic absorption spectrometry. The bioaccessibility of trace metals was assessed using the contents of trace metals in bionic extracts. The results showed that the contents of Fe, Cu, Zn and Cd in gastric juice extracts of in vitro digestion on oysters were higher than those in intestinal juice extracts. The contents of Fe and Cu in gastric juice extracts of in vitro digestion on clam were not obviously different with that of in intestinal juice extracts. Otherwise, the contents of Zn and Cd in gastric juice extracts were much higher than those of in intestinal juice extracts. The bioaccessibility of Fe, Cu, Zn and Cd in oysters using in vitro digestion was 60.2%, 83.6%, 83.1% and 76.8%, respectively. The bioaccessibility of Fe, Cu, Zn and Cd in clams was 46.3%, 86.3%, 85.3% and 87.7%, respectively. The results will provide a scientific basis for further researches such as bioaccumulation and bioavailability of trace metal in oysters and clams, evaluation of the health risk of Cd from oyster and clam consumption.

关 键 词：体外仿生消解 生物可给性 原子光谱法 微量元素 牡蛎和蛤仔 

分 类 号：O657.31[理学—分析化学] TS254.7[理学—化学]