基于氧化石墨烯荧光适体传感器的胰岛素检测  

An Aptamer-based Fluorescence Biosensor for Insulin Detection Based on Graphene Oxide

作　　者：姜利英[1] 肖小楠 周鹏磊 张培[1] 闫艳霞[1] 姜素霞[1] 陈青华[1] 

机构地区：[1]郑州轻工业学院电气信息工程学院,郑州450002

出　　处：《分析化学》2016年第2期310-314,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.61002007);河南省科技创新人才计划(No.124100510001);郑州轻工业学院2014年度研究生科技创新基金资助项目(No.2014010);郑州轻工业学院博士科研基金资助项目(No.2014BSJJ041)~~

摘　　要：采用荧光基团(FAM)标记的核酸适体作为识别元件,氧化石墨烯为淬灭剂,建立了一种高选择性、高灵敏度的核酸适体传感器。核酸适体与氧化石墨烯结合后,荧光淬灭,此时溶液无荧光;加入胰岛素后,溶液中荧光得到恢复。利用荧光分析法检测加入胰岛素前后,溶液中荧光强度的变化,获取了荧光适体传感器的线性度和灵敏度,实现对胰岛素浓度的测定。结果表明,在5×10^(-8)~1×10^(-5)mol/L范围内,胰岛素的浓度与溶液中荧光强度有良好的线性关系,检出限为10 nmol/L。采用此方法检测胰岛素,操作简便,检测速度快,准确性高,选择性好,检出限低。By using fluorophore （FAM） labeled aptamers as recognition elements and graphene oxide as a quencher, a highly selective and sensitive sensor was constructed for the fast and accurate detection of insulin. After the aptamer binding with graphene oxide, fluorescence will be quenched and fluorescence intensity disappeared; after addition of insulin, the solution fluorescence was restored. Based on the fact, we established a method for the determination of insulin concentration by measuring the fluorescence intensity. The results showed that the concentration of insulin in the range of 5× 104 - 1 × 10-5 mol/L had a good linear relationship with the fluorescence intensity. The detection limit was 10 nmol/L. This method of detecting insulin had obvious advantages of fast detection speed, high selectivity and low detection limit.

关 键 词：荧光基团 核酸适体 氧化石墨烯 荧光适体传感器 胰岛素 

分 类 号：R446[医药卫生—诊断学] TP212[医药卫生—临床医学]