广西壮药鸡骨草多糖含量测定  

Content determination of polysaccharide from Guangxi Zhuang Medicine Abrus cantoniensis Hance

作　　者：秦建鲜 莫璐[3] 黄锁义[1,4] 林梦瑶[3] 

机构地区：[1]右江民族医学院药学院,广西百色533000 [2]广西中医药大学药学院,广西南宁530001 [3]右江民族医学院临床医学院,广西百色533000 [4]右江流域特色民族药研究广西高校重点实验室,广西百色533000

出　　处：《微量元素与健康研究》2016年第2期44-47,共4页Studies of Trace Elements and Health

基　　金：广西自然科学基金资助项目(2013GXNSFAA019240);国家中医药管理局"十二五"中医药重点学科中药化学建设项目(国中医药人教发[2012]32号);广西重点学科药物化学建设项目(桂教科研[2013]16号)

摘　　要：目的：测定广西壮药鸡骨草总多糖（PACL）、90％醇沉多糖（PACLl）、70％醇沉多糖（PACL2）、40％醇沉多糖（PACL3）等多糖的含量。方法：用传统的水提法提取鸡骨草多糖，并用三氯乙酸法去除蛋白纯化。所得鸡骨草多糖采用分步醇沉法，用90％、70％、40％的乙醇将鸡骨草粗多糖进行分级沉淀，分别得到PACL和PACL1、PACL2、PACL3三个级分。通过苯酚一硫酸法显色，以葡萄糖标准液绘制标准曲线。结果：回归方程为：Y=13．514X＋0．0918，线性系数R2=0．9993，鸡骨草各多糖组分PACLl，PACL2，PACL3，PACL的含量分别为19．78％、11．19％，7．72％，4．44％，即各组分含量关系为：PACL1〉PACL2〉PACL3〉PACL。标准品的平均回收率为：98．83％～100．13％，RSD=1．24％。结论：鸡骨草多糖中多糖的含量均较高，高浓度乙醇沉淀得到的鸡骨草多糖含量较高，低浓度乙醇沉淀的鸡骨草多糖的含量较低，而且鸡骨草总糖未经过醇沉的纯化筛选，其多糖的浓度含量相对较小。Objective ：To determine the total content of polysaecharide from Guangxi Zhuang Medicine Abrus cantoniensis Hance （PACL）, 90 % polysaccharides of alcohol sink（PACL1 ）, 70 % polysaccharide of alcohol sink（PACL2）, 40 % polysaccharide of alcohol sink（PACL3）, etc. Methods： Extract polysaccharide from Guangxi Zhuang Medicine Abrus cantoniensis Hance by traditional water extraction, remove protein purification by trichloroacetic acid, With 90%, 70%, 40% ethanol to fractional precipitate the crude polysaccharide from Abrus cantoniensis Hance respectively by PACL and PACL1, PACL2, PACL3 grade three points. Use Sulfuric acid - phenol method to color development, with standard curve is drawn in the glucose standard solution. Results： Regression equation is： Y = 13. 514 ＋ 0. 0918 X, linear coefficient R2 = 0. 9993, the content of polysaccharide from every component of Abrus cantoniensis Hance PACL1, PACL2, PACL3, PACL are 19.78 %, 11.19 %, 7.72 %, 4.44 % , the relationship between each component content as follows： PACL1 〉 PACL2 〉 PACL3 〉 PACL. The standard of the average recovery is： 98.83% -- 100.13%, RSD = 1. 24 %. Conclusion（s）：Abrus cantoniensis Hanee is full of high Content of polysaccharide, high concentration of ethanol to precipitate the content of Abrus cantoniensis Hance＇ s polysaccharide is higher, low concentration of ethanol to precipitate the content of Abrus eantoniensis Hance＇ s polysaecharide is lower, in addition, the total content of polysaccbaride from Abrus cantoniensis Hance is not remove purification by alcohol precipitation, the concentration and content of the poiysaccharide are relatively lower.

关 键 词：鸡骨草 多糖 硫酸一苯酚法 分步醇沉法 

分 类 号：TQ461[化学工程—制药化工]