烟草亲和性解钾PGPR菌株筛选及其促生效果研究  

Screening of affinity PGPRs from tobacco root and their growth-promotion effects on tobacco

作　　者：龚文秀[1] 曹媛媛[1] 倪海婷[1] 孙乐妮[1] 唐欣昀[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230000

出　　处：《中国烟草学报》2016年第1期55-63,共9页Acta Tabacaria Sinica

基　　金：国家自然基金(No.41401269);安徽省高校青年基金重点项目(No.2013SQRL015ZD);安徽农业大学稳定和引进人才基金(No.yj2011-25);安徽农业大学校青年科研基金(No.2012zr006)

摘　　要：【目的】为筛选具有烟草亲和性的解钾PGPR菌株,考察PGPR对烟草生长和叶片钾含量的作用。【方法】采用解钾细菌培养基和烟草凝集素双重筛选技术,从健康烟草幼苗根际土壤中筛选具有高促生潜力的烟草亲和性解钾PGPR,测定菌株解钾能力,选取优良解钾菌株进行盆栽接种试验,调查PGPR对烟株生长影响,并测定叶片含钾量。【结果】烟草亲和性细菌占全部菌株数量的84.11%;18株PGPR的解钾能力、产IAA、产铁载体能力较强;其中7株PGPR能显著促进烟草幼苗根系生长发育,促进根系和地上部分干物质量积累,具有较大的促生长潜力。11株PGPR能促进植株对钾素的吸收,使烟草叶片含钾量显著高于施用同样水平钾肥的对照,达到施用100%钾肥处理的水平。经综合评价,TK11、TK32、TK54、TK84、TK89共5株高效烟草亲和性菌株具有进一步研究和应用的潜力。5株高效PGPR经鉴定分属5个属,具有丰富的多样性。【结论】烟草凝集素可作为筛选高效专用PGPR的有效工具,获得的5株优良PGPR为研发烟草专用生物钾肥提供了可靠材料。[Objective] This paper aims to screen affinity potassium-solubilizing bacteria PGPR strains from tobacco, and to examine their effects on growth and potassium content in leaf tobacco. [Method] Potassium-solubilizing bacteria culture and tobacco lectin methods were applied, and tobacco affinity potassium-solubilizing strains with a high potential for growth-promoting were selected from healthy tobacco seedlings rhizosphere soil. Strains with excellent potassium-solubilizing capability were selected by the potassium-solubilizing capability. Effects of the strains on growth and potassium content in leaf tobacco were investigated with pot experiment. [Result] The number of tobacco-affinity bacteria accounted for 84.11% of all isolates; 18 PGPR had strong capacity to solubilize potassium, and produced IAA and siderophore. Seven PGPR strains significantly promoted root growth and the accumulation of root and aerial parts dry matter of tobacco seedling. Eleven PGPR strains helped plant absorb more potassium, substantially increase potassium content in tobacco leaves. Potassium contents in tobacco leaves treated with these strains were much higher than those of the control with same level of potassium fertilizer dose, and even higher than those of control with 100% potassium fertilizer application. Being evaluated comprehensively with the parameters of the promotion on growth and potassium content of tobacco leaf and other characteristics, TK11, TK32, TK54, TK84 and TK89 were considered efficient affinity tobacco strains, and had the potential for further research and applications. Five efficient PGPR were identified and categorized into 5 genera. [Conclusion] Tobacco lectin can be used as an effective tool for screening high-efficiency tobacco PGPR, and 5 excellent PGPR obtained provide a reliable material for the development of the tobacco-specific potassium-solving fertilizer.

关 键 词：烟草 解钾能力 亲和性 PGPR 叶片钾含量 

分 类 号：S572[农业科学—烟草工业]